Rabbit Anti-KITLG Recombinant Antibody (VS3-CJ779) (CAT#: VS3-CJ779)
This product is a rabbit antibody that recognizes human KITLG.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/1515_E4_1_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/1515_E4_1_selected.jpg
Figure 2 IHC staining of human bone marrow
(Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_5_4_selected.jpg
(Immunohistochemical staining of human bone marrow shows nuclear positivity in hematopoietic cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_5_4_selected.jpg
Figure 3 Spleen
(Cells in red pulp
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_7_4.jpg
(Cells in red pulp
Staining: Low
Intensity: Moderate
Quantity: <25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70395/159850_B_7_4.jpg
Figure 4 RNA cell line category: Cell line enhanced (CACO-2, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049130-KITLG
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000049130-KITLG
Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human KITLG
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% Sodium Azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry.
Target
- Alternative Names
- SF; MGF; SCF; SLF; DCUA; FPH2; FPHH; KL-1; Kitl; WS2F; SHEP7; DFNA69
- Gene ID
- 4254
- UniProt ID
- P21583
- Sequence Similarities
- Belongs to the SCF family.
- Cellular Localization
- Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Secreted
- Post Translation Modifications
- A soluble form (sKITLG) is produced by proteolytic processing of isoform 1 in the extracellular domain.
Found in two differentially glycosylated forms, LMW-SCF and HMW-SCF. LMW-SCF is fully N-glycosylated at Asn-145, partially N-glycosylated at Asn-90, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97 or Asn-118. HMW-SCF is N-glycosylated at Asn-118, Asn-90 and Asn-145, O-glycosylated at Ser-167, Thr-168 and Thr-180, and not glycosylated at Asn-97.
A soluble form exists as a cleavage product of the extracellular domain.
- Protein Refseq
- NP_000890.1; NP_003985.2
- Function
- Ligand for the receptor-type protein-tyrosine kinase KIT. Plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. KITLG/SCF binding can activate several signaling pathways. Promotes phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase, and subsequent activation of the kinase AKT1. KITLG/SCF and KIT also transmit signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. KITLG/SCF and KIT promote activation of STAT family members STAT1, STAT3 and STAT5. KITLG/SCF and KIT promote activation of PLCG1, leading to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KITLG/SCF acts synergistically with other cytokines, probably interleukins.
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KITLG"
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-601 | Recombinant Anti-human KITLG Antibody | WB, IF, FuncS | IgG |
| MHH-601 | Recombinant Human Anti-human KITLG Antibody | ELISA, WB, IF, FuncS | IgG |
| MOB-1398CT | Recombinant Mouse anti-Human KITLG Monoclonal antibody (2.3_3I6-2D20) | ELISA | |
| HPAB-0299CQ | Human Anti-KITLG Recombinant Antibody (clone A2-G8) | ELISA, FC, Neut | Human IgG |
| MRO-1369-CN | Recombinant Rabbit Anti-KITLG Monoclonal Antibody (CBACN-497) | WB, IF, IHC, FC | Rabbit IgG |
Fab Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-601-F(E) | Recombinant Anti-human KITLG Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| MHH-601-F(E) | Recombinant Human Anti-human KITLG Antibody Fab Fragment | FC, ELISA, FuncS | Fab |
| HPAB-0299CQ-F(E) | Human Anti-KITLG Recombinant Antibody (clone A2-G8); Fab Fragment | ELISA, FC, Neut | Human Fab |
scFv Fragment Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-601-S(P) | Recombinant Anti-human KITLG Antibody scFv Fragment | FC, Neut, Biosensors, FuncS | scFv |
| MHH-601-S(P) | Recombinant Human Anti-human KITLG Antibody scFv Fragment | RIA, ELISA, FuncS | scFv |
| HPAB-0299CQ-S(P) | Human Anti-KITLG Recombinant Antibody (clone A2-G8); scFv Fragment | ELISA, FC, Neut | Human scFv |
Mouse Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-0475CL | Mouse Anti-KITLG Recombinant Antibody (TAB-0475CL) | ELISA, Block, FuncS | Mouse IgG |
| TAB-0475CL-S(P) | Mouse Anti-KITLG Recombinant Antibody; scFv Fragment (TAB-0475CL-S(P)) | ELISA, Block, FuncS | Mouse scFv |
| TAB-0475CL-F(E) | Mouse Anti-KITLG Recombinant Antibody; Fab Fragment (TAB-0475CL-F(E)) | ELISA, Block, FuncS | Mouse Fab |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1949 | Hi-Affi™ Recombinant Rabbit Anti-KITLG Monoclonal Antibody (DS1949AB) | FC, ICC/IF, IHC-P, WB | IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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