Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ33) (CAT#: VS3-CJ33)
This product is a mouse antibody that recognizes KLF4.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/110_C2_1_selected.jpg
Figure 2 High expression in esophagus
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
(Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_B_4_1.jpg
Figure 3 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_3.jpg
Figure 4 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_7_5.jpg
Figure 5 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: Low Intensity: Weak)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2926/8748_A_4_6.jpg
Figure 6 RNA cell line category: Cell line enhanced (ASC TERT1, hTCEpi, hTERT-HME1, OE19, U-2197)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000136826-KLF4
Specifications
- Immunogen
- Recombinant full length of mouse KLF4
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human and Mouse KLF4
- Species Reactivity
- Human, Mouse
- Applications
- WB, ELISA
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein G affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Concentration
- 1 mg/mL (lot specific)
- Buffer
- 50% Glycerol, 1% BSA, PBS, pH7.4.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in ELISA (1:5000-1:20000), Western Blot (1:100-1:2000).
Target
- Alternative Names
- EZF; GKLF
- Gene ID
- 9314
- UniProt ID
- O43474
- Sequence Similarities
- Belongs to the krueppel C2H2-type zinc-finger protein family.
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Ubiquitinated. 'Lys-48'-linked ubiquitinated and targeted for proteasomal degradation by the SCF(BTRC) E3 ubiquitin-protein ligase complex, thereby negatively regulating cell pluripotency maintenance and embryogenesis.
Polyglutamylated by TTLL1 and TTLL4 at Glu-411, which inhibits KLF4 binding with E3 ligase component BTRC, thereby impeding ubiquitination. Deglutamylated by CCP1 and CCP6; deglutamylation promotes KLF4 ubiquitination. KLF4 glutamylation state plays a critical role in the regulation of its function in cell reprogramming, pluripotency maintenance and embryogenesis.
- Protein Refseq
- NP_001300981.1; NP_004226.3
- Function
- Transcription factor; can act both as activator and as repressor. Binds the 5'-CACCC-3' core sequence. Binds to the promoter region of its own gene and can activate its own transcription. Regulates the expression of key transcription factors during embryonic development. Plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. Required for establishing the barrier function of the skin and for postnatal maturation and maintenance of the ocular surface. Involved in the differentiation of epithelial cells and may also function in skeletal and kidney development. Contributes to the down-regulation of p53/TP53 transcription.
Recommended Products
Isotype Control
Secondary Antibody
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "KLF4"
Select a product category from the dropdown menu below to view related products.
Please select product type
IgG Antibody Products
Rabbit Monoclonal Antibody Products
Antibody Magnetic Beads
Cancer Stem Cell Biomarker Specific Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1026CT | Recombinant Mouse anti-Human KLF4 Monoclonal antibody (2F6) | ELISA, ICC/IF, IHC-P, WB | |
| MRO-1997-CN | Rabbit Anti-KLF4 Polyclonal Antibody (MRO-1997-CN) | WB, IHC, FC | Rabbit IgG |
| ZG-0786J | Rabbit Anti-KLF4 Recombinant Antibody (clone 3A1) | ELISA, WB, IHC, IF, IP | Rabbit IgG |
| VS3-CJ149 | Mouse Anti-KLF4 Recombinant Antibody (VS3-CJ149) | WB | Mouse IgG1, κ |
| VS7-0425-WR228 | Mouse Anti-KLF4 Monoclonal Antibody (VS7-0425-WR228) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
Customer Reviews and Q&As
There are currently no Customer reviews or questions for VS3-CJ33. Click the button above to contact us or submit your feedback about this product.
Popular products with customers
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA
Application: Neut, ELISA, Inhib, ICC, WB
Application: Neut, FC, IHC-Fr, IP, BA
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: IF, IP, Neut, FuncS, ELISA, FC
Application: ELISA, Neut, FuncS
For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
