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    Mouse Anti-KRT18 Monoclonal Antibody (VS7-0425-WR254) (CAT#: VS7-0425-WR254)

    This product is a mouse IgG antibody that binds to human KRT18. The antibody recognizes specific amino acid domain of human KRT18 (aa391-483). It can be used for WB, IHC-P, IF, and ELISA assays.

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line U-2 OS
    Figure 1 IF staining of human cell line U-2 OS
    (Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
    Figure 2 IHC staining of human placenta
    Figure 2 IHC staining of human placenta
    (Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
    Normal Tissue
    Figure 5 Colon
    Figure 5 Colon
    (Endocrine cells
    Staining:

    High

    Intensity: Strong

    Enterocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%

    Goblet cells
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
    Figure 6 Liver
    Figure 6 Liver
    (Cholangiocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous
    Hepatocytes
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/
    membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
    Figure 7 Kidney
    Figure 7 Kidney
    (Collecting ducts
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Distal tubules
    Staining:

    High

    Intensity: Strong
    Quantity:>75%)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
    Figure 8 Placenta
    Figure 8 Placenta
    (Cytotrophoblasts
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Endothelial cells
    Staining:

    High

    Intensity: Strong
    Quantity: 75%-25%
    Hofbauer cells
    Staining:

    High

    Intensity: Strong
    Quantity: 75%-25%
    Syncytiotrophoblasts - cell body
    Staining:

    High

    Intensity: Strong
    Quantity:>75%
    Syncytiotrophoblasts - microvilli
    Staining:

    High

    Intensity: Strong)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
    RNA Expression
    Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
    Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
    Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
    Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18

    Specifications

    • Immunogen
    • Recombinant human KRT18 (AA: 391-483) fragment expressed in E. Coli.
    • Host Species
    • Mouse
    • Type
    • Mouse IgG
    • Specificity
    • Human KRT18
    • Species Reactivity
    • Human
    • Molecule Class
    • Type I Keratin Family
    • Applications
    • WB, IHC-P, IF, ELISA
    • Conjugate
    • Unconjugated
    • Related Disease
    • Cirrhosis (CIRRH)

    Product Property

    • Clonality
    • Monoclonal
    • Purity
    • >95% as determined by SDS-PAGE and SEC-HPLC analysis
    • Format
    • Liquid
    • Concentration
    • 1 mg/ml
    • Preservative
    • 0.03% sodium azide
    • Storage
    • Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
    • Shipping
    • Ice packs

    Applications

    • Application Notes
    • Western Blot: 1:500 - 1:2000
      Immunohistochemistry: 1:200 - 1:1000
      Enzyme-linked Immunosorbent Assay: 1:10000

    Target

    • Alternative Names
    • K18; CK-18; CYK18
    • Long Name
    • Keratin 18
    • Cellular Localization
    • Cytoplasm; Intermediate filament; Keratin; Nucleus
    • Post Translation Modifications
    • Acetylation; Glycoprotein; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
    • Function
    • Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity).; When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.

    Recommended Products

    Isotype Control

    Secondary Antibody

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "KRT18"

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    CAT Product Name Application Type
    BRD-0318MZ Chicken Anti-KRT18 Polyclonal IgY WB Chicken antibody

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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