Mouse Anti-KRT18 Recombinant Antibody (clone 3G6E4) (CAT#: VS7-0425-WR252)
This product is a mouse IgG1 antibody that binds to human KRT18. The antibody 3G6E4 recognizes specific amino acid domain of human KRT18 (AA: 1-150). It can be used for WB, IHC, ICC, and FC assays.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/if_selected.jpg
Figure 2 IHC staining of human placenta
(Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
(Immunohistochemical staining of human placenta shows distinct membranous and cytoplasmic positivity in trophoblastic cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/ihc_selected.jpg
Figure 3 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/21_G9_1_blue_red_green.jpg
Figure 4 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/1605/22_G9_1_blue_red_green.jpg
Figure 5 Colon
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
(Endocrine cells
Staining:
High
Intensity: Strong
Enterocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Goblet cells
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_8_3.jpg
Figure 6 Liver
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
(Cholangiocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous
Hepatocytes
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/
membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_9_4.jpg
Figure 7 Kidney
(Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
(Collecting ducts
Staining:
High
Intensity: Strong
Quantity:>75%
Distal tubules
Staining:
High
Intensity: Strong
Quantity:>75%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_7_5.jpg
Figure 8 Placenta
(Cytotrophoblasts
Staining:
High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:
High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:
High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
(Cytotrophoblasts
Staining:
High
Intensity: Strong
Quantity:>75%
Endothelial cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Hofbauer cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Syncytiotrophoblasts - cell body
Staining:
High
Intensity: Strong
Quantity:>75%
Syncytiotrophoblasts - microvilli
Staining:
High
Intensity: Strong)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30/252_A_3_7.jpg
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
Figure 9 RNA cell line category: Cell line enhanced (A549, BEWO, CACO-2, CAPAN-2, MCF7, SK-BR-3)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000111057-KRT18
Specifications
- Immunogen
- Recombinant human KRT18 (AA: 1-150) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human KRT18
- Species Reactivity
- Human
- Clone
- 3G6E4
- Molecule Class
- Type I Keratin Family
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cirrhosis (CIRRH)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- K18; CK-18; CYK18
- Gene ID
- 3875
- UniProt ID
- P05783
- Long Name
- Keratin 18
- Cellular Localization
- Cytoplasm; Intermediate filament; Keratin; Nucleus
- Post Translation Modifications
- Acetylation; Glycoprotein; Isopeptide bond; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_000215.1
- Function
- Involved in the uptake of thrombin-antithrombin complexes by hepatic cells (By similarity).; When phosphorylated, plays a role in filament reorganization. Involved in the delivery of mutated CFTR to the plasma membrane. Together with KRT8, is involved in interleukin-6 (IL-6)-mediated barrier protection.
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1264z | Mouse Anti-KRT18 Recombinant Antibody (clone 21C5) | FC, IF, IHC, WB | Mouse IgG1 |
| MRO-0441-CN | Recombinant Mouse Anti-KRT18 Monoclonal Antibody (6-19) | WB, IF, IHC | Mouse IgG2a |
| ZG-0789F | Mouse Anti-KRT18 Recombinant Antibody (clone DC10) | IHC, WB | Mouse IgG1 |
| VS3-XY994 | Mouse Anti-KRT18 Recombinant Antibody (clone 2B5-B6-F7) | FC, IF | Mouse IgG2a |
| VS3-XY995 | Mouse Anti-KRT18 Recombinant Antibody (clone 4D11E4) | ELISA, WB, IHC | Mouse IgG2b |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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