Mouse Anti-LEF1 Recombinant Antibody (clone 1A4B11)
CAT#: VS7-0425-WR282
This product is a mouse IgG1 antibody that binds to human LEF1. The antibody 1A4B11 recognizes specific amino acid domain of human LEF1 (AA: 33-138). It can be used for WB and IHC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2087/34_A1_2_selected.jpg
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Specifications
- Immunogen
- Recombinant human LEF1 (AA: 33-138) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human LEF1
- Species Reactivity
- Human
- Clone
- 1A4B11
- Molecule Class
- High-Mobility Group (HMG) Box Superfamily
- Applications
- WB, IHC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- LEF1; TCF7L3; LEF-1; T Cell-Specific Transcription Factor 1-Alpha; Lymphoid Enhancer-Binding Factor 1; TCF1-Alpha; TCF10
- Gene ID
- 51176
- UniProt ID
- Q9UJU2
- Long Name
- Lymphoid enhancer-binding factor 1
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001124185.1
- Function
- Activator; DNA-binding
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "LEF1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2035 | Hi-Affi™ Recombinant Rabbit Anti-LEF1 Monoclonal Antibody (DS2035AB) | FC, ICC, IF, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0693F | Mouse Anti-LEF1 Recombinant Antibody (clone 1C3) | WB | Mouse IgG1 |
| ZG-0694F | Mouse Anti-LEF1 Recombinant Antibody (clone 1C3.1D10) | WB | Mouse IgG1 |
| ZG-0714F | Mouse Anti-LEF1 Recombinant Antibody (clone 3A12) | WB | Mouse IgG1 |
| VS3-WK1203 | Mouse Anti-LEF1 Recombinant Antibody (VS3-WK1203) | WB | Mouse IgG1, κ |
| VS7-0425-WR283 | Mouse Anti-LEF1 Recombinant Antibody (clone 3G5F9) | IHC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY3979 | Anti-LEF1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY3980 | Anti-Mouse LEF1 Immunohistochemistry Kit | IHC |
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