Anti-LGR5 Antibody Prodrug, Protease Activated (VS-1025-YC156)
CAT#: VS-1025-YC156
The Anti-LGR5 Antibody Prodrug, Protease Activated (VS-1025-YC156) is an antibody prodrug comprised of a recombinant mouse antibody targeting human LGR5, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to LGR5 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to LGR5 effectively. LGR5 (Leucine-rich repeat-containing G protein-coupled receptor 5) is a marker for cancer stem cells and is often overexpressed in various cancers, particularly colorectal and hepatocellular carcinoma. It acts as a positive regulator of Wnt signaling and is associated with tumor initiation, recurrence, and metastasis.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
❮
❯
Specifications
- Host Animal
- Mouse
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human LGR5
- Target
- LGR5
- Species Reactivity
- Human
- Isotype
- IgG
- Epitope
- LGR5-ECD (aa 22-557)
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
- Alternative Names
- FEX; HG38; GPR49; GPR67; GRP49
- Gene ID
- 8549
- UniProt ID
- O75473
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-1025-YC156. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "LGR5"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1650-VHH | Recombinant Anti-Human LGR5 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2770z | Mouse Anti-LGR5 Recombinant Antibody (clone 44A6) | FC, ICC, FuncS | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-490CL | Human Anti-LGR5 Recombinant Antibody (TAB-490CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-196CT | Rabbit Anti-LGR5 Recombinant Antibody (TAB-196CT) | IHC, FC, ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-197CT | Rabbit Anti-LGR5 Recombinant Antibody (TAB-197CT) | IHC, FC, ELISA, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-198CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-198CT) | ELISA, FC, RIA, IP, WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT | Human Anti-LGR5 Recombinant Antibody (TAB-199CT) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-200CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-200CT) | ELISA, FC, RIA, IP, WB | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-201CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-201CT) | IF, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-199CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (TAB-199CT-F(E)) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2048 | Hi-Affi™ Recombinant Rabbit Anti-LGR5 Monoclonal Antibody (DS2048AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0101-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0102-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0102-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0103-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0103-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0104-CN | Human Anti-LGR5 Recombinant Antibody (HPAB-0104-CN) | WB, ELISA, FC, IHC | Humanized IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0101-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0102-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0102-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0103-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0103-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0104-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0104-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0106-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0106-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0101-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0102-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0102-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0103-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0103-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0104-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0104-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0106-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0106-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY32 | Mouse Anti-LGR5 Agonistic Antibody (VS-0724-XY32) | FC, Agonistic assays, CyTOF®, ICC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY33 | Rat Anti-Lgr5 Agonistic Antibody (VS-0724-XY33) | FC, Agonistic assays, CyTOF®, ICC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY173 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0424-XY173) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC625 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0724-YC625) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC71 | Rabbit Anti-LGR5 Antibody (VS-0924-YC71) - Cancer Stem Cell Marker | IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC634 | Recombinant Anti-LGR5 Vesicular Antibody, EV Displayed (VS-0425-YC634) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4011 | Anti-LGR5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4012 | Anti-Mouse LGR5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC118 | Recombinant Anti-LGR5 (AA 22-322 x AA 323-558) Biparatopic Antibody, Tandem scFv | ELISA, FC, IHC, WB | Tandem scFv |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, FC, IP, ELISA, Neut, FuncS, IF
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FuncS, IF, Neut, ELISA, FC, IP, IHC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: IF, IP, Neut, FuncS, ELISA, FC, WB
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: FuncS, IF, Neut, ELISA, FC, IP, WB
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: WB, IHC, FC, Cyt, ELISA
Application: ELISA, Neut, FuncS
Application: ELISA, IP, WB, IHC, IF, FuncS
Application: Neut, ELISA, Inhib, ICC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
