Rabbit Anti-LGR5 Antibody (VS-0924-YC71) - Cancer Stem Cell Marker
CAT#: VS-0924-YC71
Introducing the VS-0924-YC71 anti-LGR5 antibody, which specifically targets the human LGR5 protein, a marker of cancer stem cells (CSCs). This antibody is ideal for detecting LGR5 in CSC tissues or cells via Immunohistochemistry, with a recommended dilution range of 1/200 to 1/500.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Neuropil
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Cells in tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
RNA Expression
Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5
❮
❯
Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human LGR5
- Species Reactivity
- Human
- Applications
- IHC
Product Property
- Clonality
- Polyclonal
- Purification
- Affinity purified using the PrEST antigen as affinity ligand
- Concentration
- 0.1 mg/mL
- Buffer
- PBS, pH 7.2, with 40% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Immunohistochemistry (IHC): 1/200-1/500
Target
- Alternative Names
- FEX; HG38; GPR49; GPR67; GRP49
- Gene ID
- 8549
- UniProt ID
- O75473
- Long Name
- Leucine-rich repeat containing G protein-coupled receptor 5
- Cellular Localization
- #Cell membrane
#Golgi apparatus
#Membrane
- Post Translation Modifications
- #Disulfide bond
#Glycoprotein
- Function
- #G-protein coupled receptor
#Receptor
#Transducer
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CITATIONS
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Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| NAB-1650-VHH | Recombinant Anti-Human LGR5 VHH Single Domain Antibody | WB, ICC, ChiP, FA, ELISA | Llama VHH |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-490CL | Human Anti-LGR5 Recombinant Antibody (TAB-490CL) | ELISA | Human IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-196CT | Rabbit Anti-LGR5 Recombinant Antibody (TAB-196CT) | IHC, FC, ELISA, WB | Rabbit IgG |
| TAB-197CT | Rabbit Anti-LGR5 Recombinant Antibody (TAB-197CT) | IHC, FC, ELISA, WB | Rabbit IgG |
| TAB-198CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-198CT) | ELISA, FC, RIA, IP, WB | Mouse IgG |
| TAB-200CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-200CT) | ELISA, FC, RIA, IP, WB | Mouse IgG1 |
| TAB-201CT | Mouse Anti-LGR5 Recombinant Antibody (TAB-201CT) | IF, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-199CT | Human Anti-LGR5 Recombinant Antibody (TAB-199CT) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| TAB-199CT-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (TAB-199CT-S(P)) | ELISA, FC, RIA, IP, WB | Mouse scFv |
| TAB-199CT-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (TAB-199CT-F(E)) | ELISA, FC, RIA, IP, WB | Chimeric antibody (mouse/human) |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2048 | Hi-Affi™ Recombinant Rabbit Anti-LGR5 Monoclonal Antibody (DS2048AB) | WB, IP, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0101-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| HPAB-0102-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0102-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| HPAB-0103-CN | Mouse Anti-LGR5 Recombinant Antibody (HPAB-0103-CN) | WB, ELISA, FC, IHC | Mouse IgG |
| HPAB-0104-CN | Human Anti-LGR5 Recombinant Antibody (HPAB-0104-CN) | WB, ELISA, FC, IHC | Humanized IgG |
| HPAB-0106-CN | Human Anti-LGR5 Recombinant Antibody (HPAB-0106-CN) | WB, ELISA, FC, IHC | Human IgG1, κ |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0101-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0102-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0102-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0103-CN-S(P) | Mouse Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0103-CN-S(P)) | WB, ELISA, FC, IHC | Mouse scFv |
| HPAB-0104-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0104-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| HPAB-0106-CN-S(P) | Human Anti-LGR5 Recombinant Antibody; scFv Fragment (HPAB-0106-CN-S(P)) | WB, ELISA, FC, IHC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0101-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0101-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0102-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0102-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0103-CN-F(E) | Mouse Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0103-CN-F(E)) | WB, ELISA, FC, IHC | Mouse Fab |
| HPAB-0104-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0104-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| HPAB-0106-CN-F(E) | Human Anti-LGR5 Recombinant Antibody; Fab Fragment (HPAB-0106-CN-F(E)) | WB, ELISA, FC, IHC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-XY32 | Mouse Anti-LGR5 Agonistic Antibody (VS-0724-XY32) | FC, Agonistic assays, CyTOF®, ICC | Mouse IgG2a |
| VS-0724-XY33 | Rat Anti-Lgr5 Agonistic Antibody (VS-0724-XY33) | FC, Agonistic assays, CyTOF®, ICC | Rat IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY173 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0424-XY173) | IP, Protein Purification | |
| VS-0724-YC625 | AbPlus™ Anti-LGR5 Magnetic Beads (VS-0724-YC625) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0425-YC634 | Recombinant Anti-LGR5 Vesicular Antibody, EV Displayed (VS-0425-YC634) | ELISA, FC, Cell-uptake |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4011 | Anti-LGR5 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4012 | Anti-Mouse LGR5 Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-YC118 | Recombinant Anti-LGR5 (AA 22-322 x AA 323-558) Biparatopic Antibody, Tandem scFv | ELISA, FC, IHC, WB | Tandem scFv |
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