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    AbPlus™ Anti-LGR5 Magnetic Beads (VS-0724-YC625) (CAT#: VS-0724-YC625)

    The AbPlus Anti-LGR5 Magnetic Beads (VS-0724-YC625) is an innovative affinity resin which is bound with anti-LGR5 specific antibody. The beads were designed for small-scale affinity purification and immunoprecipitation (IP) of LGR5 protein under native and denaturing conditions.

    Specific Inquiry
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location and Protein Expression
    Figure 1 IF staining of human cell line Hep G2
    Figure 1 IF staining of human cell line Hep G2
    (Immunofluorescent staining of human cell line Hep G2 shows localization to nucleoplasm & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/1404_H2_1_selected.jpg
    Figure 2 IHC staining of human colon
    Figure 2 IHC staining of human colon
    (Immunohistochemical staining of human colon shows cytoplasmic positivity in glandular cells.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/ihc_selected.jpg
    Figure 3 IF staining of human cell line A-431
    Figure 3 IF staining of human cell line A-431
    (Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & the Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/103_B2_1_blue_red_green.jpg
    Figure 4 IF staining of human cell line U-251 MG
    Figure 4 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to Golgi apparatus.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/102_B2_1_blue_red_green.jpg
    Normal Tissue
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glial cells
    Staining:Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Cytoplasmic/membranous nuclear
    Neuropil
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_B_7_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Glandular cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Peripheral nerve/ganglion
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Cholangiocytes
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in glomeruli
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous
    Cells in tubules
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_8_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Leydig cells
    Staining:Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/12530/30616_A_6_6.jpg
    RNA Expression
    Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
    Figure 10 RNA cell line category: Group enriched (Hep G2, SH-SY5Y)
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000139292-LGR5

    Specifications

    • Applications
    • Immunoprecipitation, Protein Purification
    • Matrix
    • Magnetic bead
    • Bead Ligand
    • Anti-LGR5 specific antibody
    • Target
    • LGR5
    • Immunogen
    • Synthetic peptide corresponding to the C-terminus of the human LGR5.
    • Target Species
    • Human
    • Bead Capacity
    • 40 mg/mL
    • Bead size
    • 25 μm
    • Format
    • Suspension
    • Concentration
    • 2 mg/mL
    • Buffer
    • PBS, pH 7.4
    • Preservative
    • 0.1% Sodium azide
    • Storage
    • Stored at 4°C, and is stable for up to 2 years. Do not centrifuge, dry or freeze the magnetic beads.

    Applications

    • Application Notes
    • The beads are in suspension and will settle upon storage. Prior to use, mix the vial gently (do not vortex) to ensure delivery of proper bead volume.

    Target

    • Introduction
    • The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.
    • Alternative Names
    • FEX; HG38; GPR49; GPR67; GRP49

    Downloads

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    CAT Product Name Application Type
    NAB-1650-VHH Recombinant Anti-Human LGR5 VHH Single Domain Antibody WB, ICC, ChiP, FA, ELISA Llama VHH

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