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Rabbit Anti-MAF Recombinant Antibody (clone R04-6K7) (CAT#: VS3-XY3423)

This antibody is a recombinant rabbit antibody targeting the MAF (MAF bZIP transcription factor), which involves in embryonic lens fiber cell development. It specifically recognizes human MAF. It also has cross-reactivity against mouse and rat MAF. It has been reported for use in Immunohistochemistry-Paraffin. The recommended dilutions of Immunohistochemistry-Paraffin is 1/50-1/100.


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Normal Tissue
Figure 1 IHC staining of human duodenum
Figure 1 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/28289/59696_A_9_2_selected.jpg
Figure 2 Cerebral cortex
Figure 2 Cerebral cortex
(Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_B_9_5.jpg
Figure 3 Colon
Figure 3 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_3.jpg
Figure 4 Liver
Figure 4 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_4.jpg
Figure 5 Kidney
Figure 5 Kidney
(Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_5.jpg
Figure 6 Testis
Figure 6 Testis
(Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_5_6.jpg
Figure 7 Lymph node
Figure 7 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Figure 8 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000178573-MAF

Specifications

  • Host Species
  • Rabbit
  • Type
  • Rabbit IgG
  • Specificity
  • Human, Mouse and Rat MAF
  • Species Reactivity
  • Human, Mouse, Rat
  • Clone
  • R04-6K7
  • Applications
  • IHC-P
  • Related Disease
  • Ayme-Gripp Syndrome; Cataract 21, Multiple Types

Product Property

  • Clonality
  • Monoclonal
  • Aggregation
  • < 5%
  • Format
  • Liquid
  • Buffer
  • PBS (pH 7.4), 150 mM NaCl and 50% glycerol
  • Preservative
  • 0.02% sodium azide
  • Storage
  • Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
  • Shipping
  • Ice packs

Applications

  • Application Notes
  • IHC: 1/50-1/100

Target

  • Alternative Names
  • CCA4; AYGRP; c-MAF; CTRCT21
  • Long Name
  • MAF bZIP transcription factor
  • Cellular Localization
  • Nucleus
  • Post Translation Modifications
  • Ubiquitinated, leading to its degradation by the proteasome.
    Ubiquitination is triggered by glucocorticoids.
    (O75444-MAF_HUMAN)
    Phosphorylated by GSK3 and MAPK13 on serine and threonine residues (Probable).
  • Function
  • Acts as a transcriptional activator or repressor.
    Involved in embryonic lens fiber cell development.
    Recruits the transcriptional coactivators CREBBP and/or EP300 to crystallin promoters leading to up-regulation of crystallin gene during lens fiber cell differentiation.
    Activates the expression of IL4 in T helper 2 (Th2) cells.

Recommended Products

Isotype Control

Recommended Dilution Buffer

Secondary Antibody

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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See other products for "MAF"

Recombinant Antibody

CAT Product Name Application Type
MOB-4079z Mouse Anti-MAF Recombinant Antibody (clone 35G11) WB, IP, IF, ELISA Mouse IgG1, κ
MOB-0267CT Recombinant Mouse anti-Human MAF Monoclonal antibody (EML558) WB
HPAB-693-FY Rabbit Anti-MAF Recombinant Antibody (HPAB-693-FY) WB, SPR Rabbit IgG

Intrabody

CAT Product Name Application Type
IAB-B020(A) Recombinant Anti-human Maf Intrabody [(D-Arg)9] IF, ELISA, FuncS scFv-(D-Arg)9
IAB-B020(G) Recombinant Anti-human Maf Intrabody [+36 GFP] FC, IF, FuncS scFv-(+36GFP)
IAB-B020(T) Recombinant Anti-human Maf Intrabody [Tat] CO-IP, FuncS scFv-Tat

Rabbit Monoclonal Antibody

CAT Product Name Application Type
MOR-2120 Hi-Affi™ Recombinant Rabbit Anti-MAF Monoclonal Antibody (DS2120AB) WB IgG

scFv Fragment Antibody

CAT Product Name Application Type
HPAB-693-FY-S(P) Rabbit Anti-MAF Recombinant Antibody; scFv Fragment (HPAB-693-FY-S(P)) WB, ELISA Rabbit scFv

Fab Fragment Antibody

CAT Product Name Application Type
HPAB-693-FY-F(E) Rabbit Anti-MAF Recombinant Antibody; Fab Fragment (HPAB-693-FY-F(E)) WB, ELISA Rabbit Fab

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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