Recombinant Anti-human Maf Intrabody [(D-Arg)9]
CAT#: IAB-B020(A)
Recombinant anti-Maf intrabody linked to (D-Arg)9 works within cells and directs against the proto-oncoprotein Maf, expressed in E. coli cells.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate nuclear and cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/28289/59696_A_9_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Neuropil Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_B_9_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Hepatocytes Staining: Low Intensity: Weak Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_8_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Leydig cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_5_6.jpg
Normal Tissue
Figure 7 Lymph node
Non-germinal center cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/10296/32682_A_9_8.jpg
RNA Expression
Figure 8 RNA cell line category: Group enriched (Karpas-707, RPMI-8226)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000178573-MAF
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Specifications
- Immunogen
- human v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog
- Host Species
- human
- Type
- scFv-(D-Arg)9
- Cell Penetrating Peptide
- (D-Arg)9
It has been proven that polyarginine peptides composed of nine arginine amino acids (Arg-9) are able to translocate across cells very efficiently. This raises the question if molecular dynamics simulations would show a similar pathway for this highly hydrophilic and charged peptide as predicted for the Tat peptide. The simplicity of Arg-9 over other CPPs also helps isolate the role of the Arg sidechain on the translocation without the effect of other sidechains. Therefore, in this work we study computationally the translocation of Arg-9 through a lipid bilayer and show that the translocation mechanism is the same as for the Tat peptide.
- Species Reactivity
- human
- Applications
- Formats of immunological tests: Immunofluorescence; Enzyme-linked Immunosorbent Assay; Functional studies
Product Property
- Purity
- >95% as determined by analysis by SDS-PAGE
- Storage
- Store the antibody (in aliquots) at -20°C. Avoid repeated freezing and thawing of samples.
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| MOB-4079z | Mouse Anti-MAF Recombinant Antibody (clone 35G11) | WB, IP, IF, ELISA | Mouse IgG1, κ |
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| CAT | Product Name | Application | Type |
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| IAB-B020(G) | Recombinant Anti-human Maf Intrabody [+36 GFP] | FC, IF, FuncS | scFv-(+36GFP) |
| IAB-B020(T) | Recombinant Anti-human Maf Intrabody [Tat] | CO-IP, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2120 | Hi-Affi™ Recombinant Rabbit Anti-MAF Monoclonal Antibody (DS2120AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-693-FY-S(P) | Rabbit Anti-MAF Recombinant Antibody; scFv Fragment (HPAB-693-FY-S(P)) | WB, ELISA | Rabbit scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-693-FY-F(E) | Rabbit Anti-MAF Recombinant Antibody; Fab Fragment (HPAB-693-FY-F(E)) | WB, ELISA | Rabbit Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4164 | Anti-MAF Immunohistochemistry Kit | IHC |
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