Rabbit Anti-MAP2K7 Recombinant Antibody (VS3-CJ125)
CAT#: VS3-CJ125
This product is a rabbit antibody that recognizes MAP2K7.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line PC-3
Immunofluorescent staining of human cell line PC-3 shows localization to plasma membrane & cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/64711/1186_B9_2_selected.jpg
Normal Tissue
Figure 2 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4262/11651_A_8_8.jpg
Normal Tissue
Figure 3 Kidney
Cells in glomeruli Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4262/11651_A_9_5.jpg
RNA Expression
Figure 4 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076984-MAP2K7
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Specifications
- Immunogen
- Recombinant protein
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat MAP2K7
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, ICC, IF, IHC, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Protein A affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 40% Glycerol, 1% BSA, TBS, pH7.4.
- Preservative
- 0.05% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry.
Target
- Alternative Names
- MEK; MKK7; JNKK2; MEK 7; MAPKK7; PRKMK7; SAPKK4; SAPKK-4
- Gene ID
- 5609
- UniProt ID
- O14733
- Sequence Similarities
- Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase subfamily.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Activated by phosphorylation on Ser-271 and Thr-275 by MAP kinase kinase kinases (MAP3Ks).
- Protein Refseq
- NP_001284484.1; NP_001284485.1; NP_660186.1
- Function
- Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K4/MKK4, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4/MKK4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The monophosphorylation of JNKs on the Thr residue is sufficient to increase JNK activity indicating that MAP2K7/MKK7 is important to trigger JNK activity, while the additional phosphorylation of the Tyr residue by MAP2K4/MKK4 ensures optimal JNK activation. Has a specific role in JNK signal transduction pathway activated by pro-inflammatory cytokines. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Part of a non-canonical MAPK signaling pathway, composed of the upstream MAP3K12 kinase and downstream MAP kinases MAPK1/ERK2 and MAPK3/ERK1, that enhances the AP-1-mediated transcription of APP in response to APOE (PubMed:28111074).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MAP2K7"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3154z | Mouse Anti-MAP2K7 Recombinant Antibody (clone 34B2) | WB, IP, IF, IHC, ELISA | Mouse IgG2a |
| MOB-1462MZ | Recombinant Mouse Anti-Human MAP2K7 Antibody | WB | Mouse antibody |
| MRO-0984-CN | Recombinant Rabbit Anti-MAP2K7 Monoclonal Antibody (CBACN-363) | WB, IF, IHC, IP, FC | Rabbit IgG |
| VS3-XY1030 | Mouse Anti-MAP2K7 Recombinant Antibody (clone 4E5) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2137 | Hi-Affi™ Recombinant Rabbit Anti-MAP2K7 Monoclonal Antibody (DS2137AB) | WB, IP, ICC, FC, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1146 | AbPlus™ Anti-MAP2K7 Magnetic Beads (VS-0724-YC1146) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4214 | Anti-MAP2K7 Immunohistochemistry Kit | IHC |
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