Mouse Anti-MITF Recombinant Antibody (VS3-CJ169)
CAT#: VS3-CJ169
This product is a mouse antibody that recognizes MITF.
Gene Expression
Normal Tissue
Figure 1 Cervix
High expression
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
Normal Tissue
Figure 2 Colon
Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
Normal Tissue
Figure 3 Kidney
Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
Normal Tissue
Figure 4 Skin
Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
Normal Tissue
Figure 5 Lymph node
Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SK-MEL-30)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
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Specifications
- Immunogen
- Synthesized peptide derived from human MITF
- Host Species
- Mouse
- Specificity
- Human MITF
- Species Reactivity
- Human
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:200-1:500).
Target
- Alternative Names
- MI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32
- Gene ID
- 4286
- UniProt ID
- O75030
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (PubMed:10587587).
Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (PubMed:10673502).
Phosphorylated in response to blue light (415nm) (PubMed:28842328).
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.
- Protein Refseq
- NP_000239.1; NP_001171896.1; NP_006713.1
- Function
- Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "MITF"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1711z | Mouse Anti-MITF Recombinant Antibody (clone 28F1) | WB, ELISA, FC, ICC, IHC | Mouse IgG1 |
| VS3-WK417 | Rabbit Anti-MITF Recombinant Antibody (clone JF100-01) | WB, ICC, FC | Rabbit IgG |
| VS3-CJ170 | Mouse Anti-MITF Recombinant Antibody (VS3-CJ170) | WB | |
| VS7-0425-WR385 | Mouse Anti-MITF Recombinant Antibody (clone 3A2E2) | FC | Mouse IgG1 |
| VS7-0425-WR386 | Mouse Anti-MITF Recombinant Antibody (clone 8F1G5) | WB, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0362MZ | Chicken Anti-MITF Polyclonal IgY | WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2236 | Hi-Affi™ Recombinant Rabbit Anti-MITF Monoclonal Antibody (DS2236AB) | WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY187 | AbPlus™ Anti-MITF Magnetic Beads (C5) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC729 | CytoStream™ Rabbit Anti-MITF Recombinant Antibody (VS13-YC729) | WB, ICC, IF, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4402 | Anti-MITF Immunohistochemistry Kit | IHC |
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