Mouse Anti-MITF Recombinant Antibody (VS3-CJ169) (CAT#: VS3-CJ169)
This product is a mouse antibody that recognizes MITF.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 Cervix
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
(High expression)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10295_B_7_3_val_selected.jpg
Figure 2 Colon
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
(Enterocytes - Mucosal lymphoid cells Staining: Medium Intensity: Strong Quantity: <25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_9_3.jpg
Figure 3 Kidney
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
(Cells in tubules Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/3259/10268_A_8_5.jpg
Figure 4 Skin
(Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
(Fibrohistiocytic cells Staining: Low Intensity: Weak Melanocytes Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_B_8_1.jpg
Figure 5 Lymph node
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
(Non-germinal center cells Staining: Low Intensity: Moderate Quantity: <25% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/2578/6353_A_8_8.jpg
Figure 6 RNA cell line category: Cell line enhanced (HMC-1, SK-MEL-30)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000187098-MITF
Specifications
- Immunogen
- Synthesized peptide derived from human MITF
- Host Species
- Mouse
- Specificity
- Human MITF
- Species Reactivity
- Human
- Applications
- IHC
- Conjugate
- Unconjugated
Product Property
- Purification
- Affinity purified
- Purity
- >95% as determined by SDS-PAGE
- Format
- Liquid
- Buffer
- 50% Glycerol, 0.5% BSA, PBS.
- Preservative
- 0.02% sodium azide
- Storage
- Store at 4°C for short term. Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- This antibody has been tested for use in Immunohistochemistry (1:200-1:500).
Target
- Alternative Names
- MI; WS2; CMM8; WS2A; COMMAD; MITF-A; bHLHe32
- Gene ID
- 4286
- UniProt ID
- O75030
- Sequence Similarities
- Belongs to the MiT/TFE family.
- Cellular Localization
- Cytoplasm, Nucleus
- Post Translation Modifications
- Phosphorylation at Ser-405 significantly enhances the ability to bind the tyrosinase promoter (PubMed:10587587).
Phosphorylated at Ser-180 and Ser-516 following KIT signaling, triggering a short live activation: Phosphorylation at Ser-180 and Ser-516 by MAPK and RPS6KA1, respectively, activate the transcription factor activity but also promote ubiquitination and subsequent degradation by the proteasome (PubMed:10673502).
Phosphorylated in response to blue light (415nm) (PubMed:28842328).
Ubiquitinated following phosphorylation at Ser-180, leading to subsequent degradation by the proteasome. Deubiquitinated by USP13, preventing its degradation.
- Protein Refseq
- NP_000239.1; NP_001171896.1; NP_006713.1
- Function
- Transcription factor that regulates the expression of genes with essential roles in cell differentiation, proliferation and survival. Binds to M-boxes (5'-TCATGTG-3') and symmetrical DNA sequences (E-boxes) (5'-CACGTG-3') found in the promoters of target genes, such as BCL2 and tyrosinase (TYR). Plays an important role in melanocyte development by regulating the expression of tyrosinase (TYR) and tyrosinase-related protein 1 (TYRP1). Plays a critical role in the differentiation of various cell types, such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium.
Recommended Products
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "MITF"
Chicken IgY Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0362MZ | Chicken Anti-MITF Polyclonal IgY | WB | Chicken antibody |
Rabbit Monoclonal Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2236 | Hi-Affi™ Recombinant Rabbit Anti-MITF Monoclonal Antibody (DS2236AB) | WB | IgG |
Recombinant Antibody
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1002-CN | Recombinant Rabbit Anti-MITF Monoclonal Antibody (JF100-01) | WB, IF, FC | Rabbit IgG |
| ZG-0774F | Mouse Anti-MITF Recombinant Antibody (clone C5) | WB, IP, IHC, GS | Mouse IgG1 |
| ZG-0782F | Mouse Anti-MITF Antibody (clone C5/D5 Cocktail) | IHC, IP, WB, GS | Mouse IgG1 |
| ZG-0788F | Mouse Anti-MITF Recombinant Antibody (clone D5) | IHC, GS, WB, IP | Mouse IgG1 |
| VS3-WK417 | Rabbit Anti-MITF Recombinant Antibody (clone JF100-01) | WB, ICC, FC | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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