Mouse Anti-MKI67 Recombinant Antibody (VS3-FY2802) (CAT#: VS3-FY2802)

• Host Species
• Mouse

• Type
• Mouse IgG

• Specificity
• Human MKI67

• Species Reactivity
• Human

• Applications
• Western Blot, Immunohistochemistry, Flow cytometry, ELISA

• Conjugate
• Unconjugated, FITC

• MW
• The molecular weight of the target protein is approximately 35.9 kDa.

• Related Disease
• Cervical Intraepithelial Neoplasia and Bowen's Disease

• Clonality
• Monoclonal Antibody

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Storage
• Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.

• Application Notes
• Western Blot: 1/500-1/2000
  Immunohistochemistry: 1/50-1/200
  Flow cytometry: 1/50-1/200
  ELISA: 1/1000-1/10000

• Alternative Names
• Marker Of Proliferation Ki-67; Antigen Identified By Monoclonal Antibody Ki-67; Protein Phosphatase 1, Regulatory Subunit 105; Antigen KI-67; Antigen Ki67; Proliferation-Related Ki-67 Antigen

• Gene ID
• 4288

• UniProt ID
• P46013

• Cellular Localization
• Chromosome, Nucleus

• Post Translation Modifications
• Phosphorylated. Hyperphosphorylated in mitosis (PubMed:10502411, PubMed:10653604).
  Hyperphosphorylated form does not bind DNA.

• Protein Refseq
• NP_001139438.1; NP_002408.3

• Function
• Required to maintain individual mitotic chromosomes dispersed in the cytoplasm following nuclear envelope disassembly (PubMed:27362226).
  Associates with the surface of the mitotic chromosome, the perichromosomal layer, and covers a substantial fraction of the chromosome surface (PubMed:27362226).
  Prevents chromosomes from collapsing into a single chromatin mass by forming a steric and electrostatic charge barrier: the protein has a high net electrical charge and acts as a surfactant, dispersing chromosomes and enabling independent chromosome motility (PubMed:27362226).
  Binds DNA, with a preference for supercoiled DNA and AT-rich DNA (PubMed:10878551).
  Does not contribute to the internal structure of mitotic chromosomes (By similarity).
  May play a role in chromatin organization (PubMed:24867636).
  It is however unclear whether it plays a direct role in chromatin organization or whether it is an indirect consequence of its function in maintaining mitotic chromosomes dispersed (Probable).

Secondary Antibody

• 2ND-040CQ
• Goat Anti-Mouse IgG (H&L) Antibody

Isotype Control

• MRO-A1591-YJ
• Mouse IgG Isotype control, Polyclonal

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