Mouse Anti-MMP2 Recombinant Antibody (clone 4D3)
CAT#: ZG-0729F
This product is a mouse antibody that recognizes MMP2.
Published Data
Gene Expression
IHC
Figure 1 Mouse Anti-MMP2 Antibody (ZG-0729F) in IHC
Immunohistochemical staining of human oesophageal adenocarcinoma tissue using MMP-2 antibody.
Murray et al., 1998
WB
Figure 2 Mouse Anti-MMP2 Antibody (ZG-0729F) in WB
Western blot using MMP-2 antibody on recombinant MMP-2 proenzyme (400 ng/lane).
Murray et al., 1998
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human stomach
Immunohistochemical staining of human stomach shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human endometrium
Immunohistochemical staining of human endometrium shows moderate cytoplasmic positivity in stromal cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_5_3_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_B_9_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_9_4.jpg
Normal Tissue
Figure 7 Gallbladder
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_4_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_8_5.jpg
Normal Tissue
Figure 9 Testis
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/2788/6952_A_6_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Non-germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/1939/7518_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Cell line enhanced (ASC diff, ASC TERT1, hTERT-RPE1, U-87 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000087245-MMP2
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Specifications
- Immunogen
- Hybridomas produced by fusion of splenocytes from BALB/c mice were immunized with a synthetic peptide derived from the C-terminus of human MMP2 protein and mouse myeloma Ag8563 cells.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Species Reactivity
- Human
- Clone
- 4D3
- Applications
- ELISA, Western Blot, Immunohistochemistry
Product Property
- Purification
- Protein A or G purified
- Storage
- Store at 4°C for short term. For long term storage, store at-20°C, avoiding freeze/thaw cycles.
Target
- Alternative Names
- Matrix Metallopeptidase 2; Matrix Metalloproteinase-2; EC 3.4.24.24; CLG4A; MMP-2; TBE-1; Matrix Metalloproteinase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metallopeptidase 2 (Gelatinase A, 72kDa Gelatinase, 72kDa Type IV Collagenase); Matrix Metalloproteinase-II; 72 KDa Type IV Collagenase
- Gene ID
- 4313
- UniProt ID
- P08253
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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