Anti-Mouse E2F1 Immunohistochemistry Kit
CAT#: VS-0525-XY2133
The E2F1 IHC kit provides complete reagents for detecting this transcription factor involved in cell cycle regulation. Suitable for paraffin and frozen tissues from various species, this kit supports research on cell proliferation, cancer, and gene expression with clear and reproducible results.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
Normal Tissue
Figure 10 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1
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Specifications
- Application
- IHC
- Size
- 50 Tests
- Species Reactivity
- Human, Mouse, Rat
- Target
- E2F1
- Primary Antibody
- Rabbit Anti-E2F1 Antibody
- Secondary Antibody
- Goat anti-Rabbit Antibody, HRP
- Sample Type
- FFPE tissue; Frozen section tissue
- Kit Storage
- All reagents should be kept at 2-8°C. The kit remains stable for up to 6 months after arrival.
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Related Diseases
Bladder Cancer
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "E2F1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0156CT | Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) | ICC, IF, IP, WB | |
| MRO-0508-CN | Rabbit Anti-E2F1 Recombinant Antibody (clone CBACN-206) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0266J | Mouse Anti-E2F1 Recombinant Antibody (ZG-0266J) | WB, ICC | Mouse IgG |
| VS3-WK839 | Rabbit Anti-E2F1 Recombinant Antibody (VS3-WK839) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-FY466 | Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-1080 | Hi-Affi™ Rabbit Anti-E2F1 Recombinant Antibody (clone DS1080AB) | WB, ICC, IF, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC333 | CytoStream™ Rabbit Anti-E2F1 Recombinant Antibody (VS13-YC333) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY2132 | Anti-E2F1 Immunohistochemistry Kit | IHC |
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