Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9)
CAT#: MOB-0156CT
Recombinant Mouse monoclonal antibody to Human E2F1, expressed in Chinese Hamster Ovary cells(CHO).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
Normal Tissue
Figure 7 Liver
Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
Normal Tissue
Figure 10 Bone marrow
Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
Normal Tissue
Figure 11 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1
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Specifications
- Immunogen
- Fusion protein containing the region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
- Host Species
- Mouse
- Species Reactivity
- Human
- Clone
- 8G9
- Applications
- Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Western Blot
- Conjugate
- Unconjugated
Product Property
- Purity
- Protein G purified
- Format
- Liquid
- Concentration
- 1.0 mg/ml
- Buffer
- Preservative: None Constituents: 10mM PBS, pH 7.4
- Storage
- Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Target
- Alternative Names
- E2F1; E2F transcription factor 1; RBP3; E2F-1; RBAP1; RBBP3; transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1;
- Gene ID
- 1869
- UniProt ID
- Q01094
- Protein Refseq
- NP_005216
- Function
- DNA binding; DNA binding; core promoter binding; cyclin-dependent protein kinase activity; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor acti
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Related Diseases
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| VS13-YC333 | CytoStream™ Rabbit Anti-E2F1 Recombinant Antibody (VS13-YC333) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
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