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    Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) (CAT#: MOB-0156CT)

    Recombinant Mouse monoclonal antibody to Human E2F1, expressed in Chinese Hamster Ovary cells(CHO).

    Specific Inquiry
    • Size:

    • Conjugation:

    • Endotoxin:

    • Purity:

    • Formats:

    • Isotype Switching:

    • Fc Engineering:

    • Modalities:

    Custom Production

    We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

    Sequences:
    Isotype :
    Purity :
    Endotoxin :
    Quantity :
    Conjugation :
    Fc Engineering :
    Modalities :
    Other Requirements:
    We require custom production
    • Gene Expression
    • Datasheet
    • MSDS
    • COA
    Subcellular Location
    Figure 1 IF staining of human cell line RH-30
    Figure 1 IF staining of human cell line RH-30
    (Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
    Figure 2 IF staining of human cell line U-251 MG
    Figure 2 IF staining of human cell line U-251 MG
    (Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
    Normal Tissue
    Figure 3 IHC staining of human rectum
    Figure 3 IHC staining of human rectum
    (Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
    Figure 4 IHC staining of human colon
    Figure 4 IHC staining of human colon
    (Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
    Figure 5 Cerebral cortex
    Figure 5 Cerebral cortex
    (Endothelial cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear
    Glial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Neuronal cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
    Figure 6 Colon
    Figure 6 Colon
    (Endothelial cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Glandular cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Peripheral nerve/ganglion
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
    Figure 7 Liver
    Figure 7 Liver
    (Hepatocytes
    Staining: Medium
    Intensity: Moderate
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
    Figure 8 Kidney
    Figure 8 Kidney
    (Cells in glomeruli
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear
    Cells in tubules
    Staining: High
    Intensity: Strong
    Quantity: 75%-25%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
    Figure 9 Testis
    Figure 9 Testis
    (Cells in seminiferous ducts
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Leydig cells
    Staining: Medium
    Intensity: Moderate
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
    Figure 10 Bone marrow
    Figure 10 Bone marrow
    (Hematopoietic cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
    Figure 11 Lymph node
    Figure 11 Lymph node
    (Germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Nuclear
    Non-germinal center cells
    Staining: High
    Intensity: Strong
    Quantity:>75%
    Location: Cytoplasmic/membranous nuclear)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
    RNA Expression
    Figure 12 RNA cell line category: Low cell line specificity
    Figure 12 RNA cell line category: Low cell line specificity
    (Cell lines ordered by descending RNA expression order.)
    * Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1

    Specifications

    • Immunogen
    • Fusion protein containing the region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
    • Host Species
    • Mouse
    • Species Reactivity
    • Human
    • Clone
    • 8G9
    • Applications
    • Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Western Blot
    • Conjugate
    • Unconjugated

    Product Property

    • Purity
    • Protein G purified
    • Format
    • Liquid
    • Concentration
    • 1.0 mg/ml
    • Buffer
    • Preservative: None Constituents: 10mM PBS, pH 7.4
    • Storage
    • Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Target

    • Alternative Names
    • E2F1; E2F transcription factor 1; RBP3; E2F-1; RBAP1; RBBP3; transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1;
    • Function
    • DNA binding; DNA binding; core promoter binding; cyclin-dependent protein kinase activity; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor acti

    Related Resources

    • Related Diseases

    Product Notes

    This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

    • Increased sensitivity
    • Confirmed specificity
    • High repeatability
    • Excellent batch-to-batch consistency
    • Sustainable supply
    • Animal-free production

    See more details about Hi-Affi™ recombinant antibody benefits.

    Downloads

    Download resources about recombinant antibody development and antibody engineering to boost your research.

    See other products for "E2F1"

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    Please select product type
    IgG Antibody Products Rabbit Monoclonal Antibody Products Flow Cytometry (FC) related Reagents and Kits: Empowering Cell Function Research
    CAT Product Name Application Type
    MOB-4001z Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) WB, IP, IF, ELISA Mouse IgG2b, κ
    MRO-0508-CN Rabbit Anti-E2F1 Recombinant Antibody (clone CBACN-206) WB, IF, IHC, IP, FC Rabbit IgG
    ZG-0641J Rabbit Anti-E2F1 Recombinant Antibody (clone 1D12) ELISA, WB, IHC, IF, FC Rabbit IgG
    VS3-QX336 Mouse Anti-E2F1 Recombinant Antibody (clone 4G8-2C2-C12) WB, IF, IP Mouse IgG2b
    VS3-WK839 Rabbit Anti-E2F1 Recombinant Antibody (VS3-WK839) WB, ICC, IF, IHC, IP, FC Rabbit IgG

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    For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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