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  • Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9)

Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) (CAT#: MOB-0156CT)

Recombinant Mouse monoclonal antibody to Human E2F1, expressed in Chinese Hamster Ovary cells(CHO).


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Specific Inquiry
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  • Conjugation:
  • Endotoxin:
  • Purity:
  • Fc Engineering:
  • Gene Expression
  • Datasheet
  • MSDS
  • COA
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Figure 1 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg
Figure 2 IF staining of human cell line U-251 MG
Figure 2 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human rectum
Figure 3 IHC staining of human rectum
(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg
Figure 4 IHC staining of human colon
Figure 4 IHC staining of human colon
(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg
Figure 5 Cerebral cortex
Figure 5 Cerebral cortex
(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg
Figure 6 Colon
Figure 6 Colon
(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg
Figure 7 Liver
Figure 7 Liver
(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg
Figure 8 Kidney
Figure 8 Kidney
(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg
Figure 9 Testis
Figure 9 Testis
(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg
Figure 10 Bone marrow
Figure 10 Bone marrow
(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg
Figure 11 Lymph node
Figure 11 Lymph node
(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg
RNA Expression
Figure 12 RNA cell line category: Low cell line specificity
Figure 12 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1

Specifications

  • Immunogen
  • Fusion protein containing the region encoding the DNA binding domain (amino acids 244-264) of E2F-1 expressed in E. coli.
  • Host Species
  • Mouse
  • Species Reactivity
  • Human
  • Clone
  • 8G9
  • Applications
  • Used for immunoassay techniques such as: Immunocytochemistry/Immunofluorescence; Immunoprecipitation; Western Blot
  • Conjugate
  • Unconjugated

Product Property

  • Purity
  • Protein G purified
  • Format
  • Liquid
  • Concentration
  • 1.0 mg/ml
  • Buffer
  • Preservative: None Constituents: 10mM PBS, pH 7.4
  • Storage
  • Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Target

  • Alternative Names
  • E2F1; E2F transcription factor 1; RBP3; E2F-1; RBAP1; RBBP3; transcription factor E2F1; PBR3; RBAP-1; RBBP-3; PRB-binding protein E2F-1; retinoblastoma-binding protein 3; retinoblastoma-associated protein 1;
  • Function
  • DNA binding; DNA binding; core promoter binding; cyclin-dependent protein kinase activity; protein binding; sequence-specific DNA binding; sequence-specific DNA binding transcription factor activity; sequence-specific DNA binding transcription factor acti

Related Resources

  • Related Diseases

Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Downloads

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Rabbit Monoclonal Antibody

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Recombinant Antibody

CAT Product Name Application Type
MRO-0508-CN Rabbit Anti-E2F1 Recombinant Antibody (clone CBACN-206) WB, IF, IHC, IP, FC Rabbit IgG
ZG-0266J Mouse Anti-E2F1 Recombinant Antibody (ZG-0266J) WB, ICC Mouse IgG
ZG-0641J Rabbit Anti-E2F1 Recombinant Antibody (clone 1D12) ELISA, WB, IHC, IF, FC Rabbit IgG
VS3-WK839 Rabbit Anti-E2F1 Recombinant Antibody (VS3-WK839) WB, ICC, IF, IHC, IP, FC Rabbit IgG
VS3-FY466 Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) WB, IHC-F, IHC-P, ICC, IF, IP Rabbit IgG

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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.

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