Hi-Affi™ Rabbit Anti-E2F1 Recombinant Antibody (clone DS1080AB) (CAT#: MOR-1080)
Recombinant Rabbit anti-E2F1 monoclonal antibody for WB, ICC/IF, IP, FC, excellent for producing superior results. Hi-Affi™ antibodies are developed from different sources, such as B cells of immunized animals, hybridoma, phage display library. The application of recombinant technology avoids the traditional ascitic fluid approach, which achieves the antibody generation in a humane way with much larger scale, greater specificity and sensitivity, and not susceptible to cell-line drift or lot-to-lot variation.
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.

(Immunofluorescent staining of human cell line RH-30 shows localization to centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/29735/1143_F6_4_selected.jpg

(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/329/951_D1_2_selected.jpg

(Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8003/ihc_selected.jpg

(Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/ihc_selected.jpg

(Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_9_5.jpg

(Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_3.jpg

(Hepatocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_4.jpg

(Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_7_5.jpg

(Cells in seminiferous ducts
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Leydig cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_4_6.jpg

(Hematopoietic cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_B_4_4.jpg

(Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/19308/43070_A_9_8.jpg

(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000101412-E2F1
Specifications
- Immunogen
- Synthetic peptide within Human E2F1 aa 350 to the C-terminus (Cysteine residue).
- Host Species
- Rabbit
- Derivation
- Phage display library
- Type
- Rabbit IgG
- Specificity
- Human E2F1
- Species Reactivity
- Mouse, Rat, Human
- Clone
- DS1080AB
- Applications
- WB, ICC, IF, IP, FC
- Conjugate
- Unconjugated
Product Property
- Purification
- Purified with Absolute Mag™ Protein A Agarose
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- Please refer to the vial label for the specific concentration.
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Target
- Alternative Names
- E2F Transcription Factor 1; Retinoblastoma-Associated Protein 1; Retinoblastoma-Binding Protein 3; PRB-Binding Protein E2F-1; RBAP-1; RBBP-3; RBBP3; E2F-1; PBR3; RBAP1; RBP3
- Gene ID
- 1869
- UniProt ID
- Q01094
Related Resources
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
See other products for "E2F1"
Select a product category from the dropdown menu below to view related products.
CAT | Product Name | Application | Type |
---|---|---|---|
MOB-4001z | Mouse Anti-E2F1 Recombinant Antibody (clone 30B9) | WB, IP, IF, ELISA | Mouse IgG2b, κ |
MOB-0156CT | Recombinant Mouse anti-Human E2F1 Monoclonal antibody (8G9) | ICC/IF, IP, WB | |
ZG-0641J | Rabbit Anti-E2F1 Recombinant Antibody (clone 1D12) | ELISA, WB, IHC, IF, FC | Rabbit IgG |
VS3-QX336 | Mouse Anti-E2F1 Recombinant Antibody (clone 4G8-2C2-C12) | WB, IF, IP | Mouse IgG2b |
VS3-FY466 | Recombinant Rabbit Anti-E2F1 Antibody (clone R03-5G3) | WB, IHC-F, IHC-P, ICC, IF, IP | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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