Rabbit Anti-MYBBP1A Recombinant Antibody (clone 3A1)
CAT#: ZG-0099U
The purified Rabbit Anti-MYBBP1A Recombinant Antibody (clone 3A1) displayed high specificity against the MYBBP1A protein of human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-MYBBP1A Antibody (ZG-0099U) in IHC
IHC image of ZG-0099U diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 2 Rabbit Anti-MYBBP1A Antibody (ZG-0099U) in IF
Immunofluorescence staining of HepG2 Cells with ZG-0099U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoli & vesicles.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/110_C3_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skin
Immunohistochemical staining of human skin shows strong nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Glial cells Staining: High Intensity: Strong Quantity:>75% Location: nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_B_7_5.jpg
Normal Tissue
Figure 4 Esophagus
Squamous epithelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_B_4_1.jpg
Normal Tissue
Figure 5 Colon
Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_A_8_3.jpg
Normal Tissue
Figure 6 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_A_7_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Sertoli cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_A_4_6.jpg
Normal Tissue
Figure 8 Lymph node
Germinal center cells Staining: Medium Intensity: Strong Quantity: <25% Location: Nuclear Non-germinal center cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5466/16124_A_7_8.jpg
RNA Expression
Figure 9 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132382-MYBBP1A
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Specifications
- Immunogen
- A synthesized peptide derived from human MYBBP1A
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human MYBBP1A
- Species Reactivity
- Human
- Clone
- 3A1
- Applications
- ELISA, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Familial Adult Myoclonic Epilepsy
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
IHC:1:50-1:200, IF:1:20-1:200
Target
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Brilliant Immunofluorescence Imaging
We used the rabbit clone 3A1 for immunofluorescence staining of nuclear proteins. The antibody specifically localized MYBBP1A, allowing us to study its interaction with DNA-binding proteins. The recommended dilution range was accurate, and we obtained sharp images with minimal background at 1:100 dilution.
Specific IHC Results
This antibody proved to be very effective for immunohistochemistry on human tissue sections. It demonstrated high specificity for the MYBBP1A protein, which is consistent with its validation data. The affinity purification clearly makes a difference in the quality of the staining we achieved in our lab.
Q&As
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What are the recommended dilutions for IHC and IF with this antibody?
A: For the Rabbit Anti-MYBBP1A Recombinant Antibody (clone 3A1), we recommend a dilution of 1:50-1:200 for Immunohistochemistry (IHC) and 1:20-1:200 for Immunofluorescence (IF). Specific conditions should be optimized by the end user.
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What is the specificity and purity of this product?
A: This antibody displays high specificity against the Human MYBBP1A protein. It is an affinity-purified Rabbit IgG recombinant antibody. The product is supplied in a buffer with glycerol and BSA to maintain stability during storage.
View the frequently asked questions answered by Creative Biolabs Support.
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "MYBBP1A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2333 | Hi-Affi™ Recombinant Rabbit Anti-MYBBP1A Monoclonal Antibody (DS2333AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1047-CN | Recombinant Rabbit Anti-MYBBP1A Monoclonal Antibody (JB40-09) | WB, FC, IHC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK294 | Rabbit Anti-MYBBP1A Recombinant Antibody (clone JB40-09) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-WK1130 | Mouse Anti-MYBBP1A Recombinant Antibody (VS3-WK1130) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1399 | Anti-MYBBP1A Immunohistochemistry Kit | IHC |
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