Rabbit Anti-NEDD8 Recombinant Antibody (VS13-YC781) (CAT#: VS13-YC781)

• Immunogen
• Recombinant human NEDD8, aa 12-57.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human and Rat NEDD8

• Species Reactivity
• Human, Rat

• Applications
• WB, ICC, IF, IHC-P, IP

• Conjugate
• NONE

• Related Disease
• Malignant Astrocytoma and Machado-Joseph Disease

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/500-1/1000
  Immunocytochemistry (ICC): 1/50-1/200
  Immunofluorescence (IF): 1/50-1/200
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/100
  Immunoprecipitation (IP): 1/50

• Alternative Names
• Neural Precursor Cell Expressed, Developmentally Down-Regulated 8; Ubiquitin-Like Protein Nedd8; Neddylin; NEDD-8; Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8;

• Gene ID
• 4738

• UniProt ID
• Q15843

• Long Name
• Neural Precursor Cell Expressed, Developmentally Down-Regulated 8

• Sequence Similarities
• Belongs to the ubiquitin family.

• Cellular Localization
• Nucleus

• Protein Refseq
• NP_006147.1

• Function
• Ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis via its conjugation to a limited number of cellular proteins, such as cullins or p53/TP53.
  Attachment of NEDD8 to cullins is critical for the recruitment of E2 to the cullin-RING-based E3 ubiquitin-protein ligase complex, thus facilitating polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
  Attachment of NEDD8 to p53/TP53 inhibits p53/TP53 transcriptional activity.
  Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M.

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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