Mouse Anti-NEFL Recombinant Antibody (clone 2F8B3)
CAT#: VS7-0425-WR462
This product is a mouse IgG1 antibody that binds to human NEFL. The antibody 2F8B3 recognizes specific amino acid domain of human NEFL (AA: 1-200). It can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line HEK 293
Immunofluorescent staining of human cell line HEK 293 shows localization to nucleoplasm, intermediate filaments & midbody.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/15021/1910_D3_1_selected.jpg
Normal Tissue
Figure 2 Low expression in tonsil
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_A_6_8_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuropil Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/14850/165171_B_8_5.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (HEK93, PC-3)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000277586-NEFL
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Specifications
- Immunogen
- Recombinant human NEFL (AA: 1-200) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NEFL
- Species Reactivity
- Human
- Clone
- 2F8B3
- Molecule Class
- Intermediate Filament Family
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NEFL; Neurofilament, Light Polypeptide; CMT2E; Neurofilament Protein, Light Chain; Light Molecular Weight Neurofilament Protein; Regulatory Subunit 110; NF68; Neurofilament Triplet L Protein; 68 KDa Neurofilament Protein; Neurofilament, Light Polypeptide
- Gene ID
- 4747
- UniProt ID
- P07196
- Long Name
- Neurofilament Light
- Cellular Localization
- Cell projection; Cytoplasm; Cytoskeleton; Intermediate filament
- Post Translation Modifications
- Acetylation; Glycoprotein; Methylation; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_006149.2
- Function
- Neurofilaments usually contain three intermediate filament proteins: NEFL, NEFM, and NEFH which are involved in the maintenance of neuronal caliber. May additionally cooperate with the neuronal intermediate filament proteins PRPH and INA to form neuronal filamentous networks (By similarity).
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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| CAT | Product Name | Application | Type |
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