Mouse Anti-NES Recombinant Antibody (clone 2C2F7) (CAT#: ZG-0156U)
Mouse anti-NES antibody (clone 2C2F7) displayed high specificity against the NES protein of Human.
Specific Inquiry
Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
WB
Figure 1 Mouse Anti-NES Antibody (ZG-0156U) in WB
Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: NES antibody at 3µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 178 kDa
Observed band size: 260 kDa
IHC
Figure 2 Mouse Anti-NES Antibody (ZG-0156U) in IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using ZG-0156U at dilution of 1:100.
IF
Figure 3 Mouse Anti-NES Antibody (ZG-0156U) in IF
Immunofluorescent analysis of U251 cells using ZG-0156U at a dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-NES Antibody (ZG-0156U) in FC
Overlay histogram showing Hela cells stained with ZG-0156U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Nestin protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NES
- Species Reactivity
- Human
- Clone
- 2C2F7
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Medulloepithelioma and Central Neurocytoma.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Target
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1043MZ | Recombinant Mouse Anti-Human NES Antibody (clone 3D2.4B22) | FC, IP WB | Mouse antibody |
| ZG-0550F | Mouse Anti-NES Recombinant Antibody (clone 10C2) | WB, IHC, IP | Mouse IgG1 |
| VS1-YJ144 | Mouse Anti-NES Recombinant Antibody (clone 10C2), FITC | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS1-YJ145 | Mouse Anti-NES Recombinant Antibody (clone 10C2), HRP | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS13-YC785 | Rabbit Anti-NES Recombinant Antibody (VS13-YC785) | WB, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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