Mouse Anti-NES Recombinant Antibody (clone 2C2F7)
CAT#: ZG-0156U
Mouse anti-NES antibody (clone 2C2F7) displayed high specificity against the NES protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Mouse Anti-NES Antibody (ZG-0156U) in WB
Positive WB detected in: U251 whole cell lysate, SH-SY5Y whole cell lysate. All lanes: NES antibody at 3µg/ml
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 178 kDa
Observed band size: 260 kDa
IHC
Figure 2 Mouse Anti-NES Antibody (ZG-0156U) in IHC
Immunohistochemistry of paraffin-embedded human kidney tissue using ZG-0156U at dilution of 1:100.
IF
Figure 3 Mouse Anti-NES Antibody (ZG-0156U) in IF
Immunofluorescent analysis of U251 cells using ZG-0156U at a dilution of 1:100 and Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-NES Antibody (ZG-0156U) in FC
Overlay histogram showing Hela cells stained with ZG-0156U (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10µg/1*106cells) for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was mouse IgG1 (10µg/1*106cells) used under the same conditions. Acquisition of>10,000 events was performed.
❮
❯
Subcellular Location
Figure 1 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/6286/1401_E3_2_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line AF22
Immunofluorescent staining of human cell line AF22 shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/1783_C4_5_selected.jpg
Subcellular Location
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to intermediate filaments.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5889/613_B1_1_selected.jpg
Normal Tissue
Figure 4 Spleen
High expression in spleen
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/7007/20723_B_8_4_val_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_7_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_7_3.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_A_8_5.jpg
Normal Tissue
Figure 8 Heart muscle
Cardiomyocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/26111/58753_B_4_6.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (AF22, HUVEC TERT2, LHCN-M2, RH-30, SuSa, WM-115)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000132688-NES
❮
❯
Specifications
- Immunogen
- Recombinant Human Nestin protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human NES
- Species Reactivity
- Human
- Clone
- 2C2F7
- Applications
- ELISA, WB, IHC, IF, FC
- Conjugate
- Unconjugated
- Related Disease
- Medulloepithelioma and Central Neurocytoma.
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:500, IF:1:50-1:200
Target
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for ZG-0156U. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "NES"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0721MZ | Chicken Anti-Nes Polyclonal IgY | ICC, IF, IHC, WB | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2422 | Hi-Affi™ Recombinant Rabbit Anti-NES Monoclonal Antibody (DS2422AB) | IHC-P, WB | IgG |
| MOR-0108-FY | Rabbit Anti-NES Recombinant Antibody (clone AFY0079) | ICC, IHC, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2064-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2064-CN) | IHC | Rabbit IgG |
| MRO-2065-CN | Rabbit Anti-NES Polyclonal Antibody (MRO-2065-CN) | IF, IHC, FC, WB | Rabbit IgG |
| VS1-YJ144 | Mouse Anti-NES Recombinant Antibody (clone 10C2), FITC | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS1-YJ145 | Mouse Anti-NES Recombinant Antibody (clone 10C2), HRP | ELISA, IF, IHC-Fr, IHC-P, WB, FC, IHC | Mouse IgG1 |
| VS3-WK1880 | Recombinant Rabbit Anti-NES Capture Antibody (clone R65-5M-1) | ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC623 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC623) | IP, Protein Purification | |
| VS-0724-YC624 | AbPlus™ Anti-NES Magnetic Beads (VS-0724-YC624) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0924-YC75 | Mouse Anti-NES Recombinant Antibody (VS-0924-YC75) - Cancer Stem Cell Marker | IHC, WB, IF | Mouse IgG1 |
| VS-0924-YC78 | Rabbit Anti-NES Antibody (VS-0924-YC78) - Cancer Stem Cell Marker | IHC, WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4792 | Anti-NES Immunohistochemistry Kit | IHC | |
| VS-0525-XY4793 | Anti-Mouse NES Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: WB, ELISA, Neut, FuncS
Application: ELISA, IHC, FC, IP, IF, Inhib
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: WB, ELISA, IHC, IP, SPR, FuncS
Application: Neut, IHC, Activ, FuncS, IF, ELISA
Application: ELISA, IHC, WB
Application: Inhib, FC, WB
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
