Mouse Anti-NHP CALR Recombinant Antibody (clone FMC 75)
CAT#: VS-1024-XY61
Employing a mouse-derived monoclonal antibody, this reagent is meticulously designed to target CALR (Calreticulin), exhibiting reactivity with hamster, human, and non-human primate specimens. The immunogen is a calreticulin-maltose binding fusion protein, enhancing specificity and binding affinity. Validated for Western blot (WB) and flow cytometry (FC) applications, this antibody offers a reliable tool for probing CALR expression and localization. Its broad reactivity and robust performance ensure its efficacy across various experimental conditions and species.
Gene Expression
Subcellular Location
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
Normal Tissue
Figure 4 Thyroid gland
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
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Specifications
- Immunogen
- Calreticulin-maltose binding fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CALR
- Species Reactivity
- Hamster, Human, Non-human primate
- Clone
- FMC 75
- Applications
- WB, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.1 mM PMSF, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, FC: 1:100
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Recommended Dilution Buffer
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- Product Name
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- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "CALR"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1130z | Mouse Anti-CALR Recombinant Antibody (clone 5H4) | ELISA, IF, IHC, WB | Mouse IgG2a |
| MOB-0287CT | Mouse Anti-CALR Recombinant Antibody (clone EML269) | IHC-P, WB | Mouse IgG1 |
| HPAB-0271-YC | Mouse Anti-CALR Recombinant Antibody (clone 8B2-H6-10.7) | FC, WB | Mouse IgG2a, κ |
| MRO-0219-CN | Mouse Anti-CALR Recombinant Antibody (clone 7B1) | WB, IHC, IF, FC | Mouse IgG1 |
| MRO-0220-CN | Mouse Anti-CALR Recombinant Antibody (clone 7B2) | WB, IHC, IF, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| BRD-0076MZ | Chicken Anti-Calreticulin Polyclonal IgY | IHC | Chicken antibody |
| BRD-0772MZ | Chicken Anti-Calreticulin Polyclonal IgY | Blocking Assay (BLOCK), IHC (Paraffin) (IHC (P)), ICC (ICC), Flow Cytometry (Flow), IHC (IHC) | Chicken antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MHC-LC1043 | PE-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM | |
| MHC-LC1044 | APC-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM | |
| MHC-LC1045 | BV421-A*02:01/Human CALR (MLLSVPLLLG) MHC Tetramer | FCM |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0443 | Hi-Affi™ Rabbit Anti-CALR Recombinant Antibody (clone DS443AB) | WB, IHC, ICC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-YC-S(P) | Mouse Anti-CALR Recombinant Antibody (clone 8B2-H6-10.7); scFv Fragment | FC, WB | Mouse scFv |
| HPAB-0446-YJ-S(P) | Mouse Anti-CALR Recombinant Antibody (clone FMMU-CRT-10); scFv Fragment | ELISA, WB, IHC, FC | Mouse scFv |
| HPAB-0447-YJ-S(P) | Mouse Anti-CALR Recombinant Antibody (clone chFMMU-CRT-10); scFv Fragment | ELISA, WB, IHC, FC | Mouse scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0271-YC-F(E) | Mouse Anti-CALR Recombinant Antibody (clone 8B2-H6-10.7); Fab Fragment | FC, WB | Mouse Fab |
| HPAB-0446-YJ-F(E) | Mouse Anti-CALR Recombinant Antibody (clone FMMU-CRT-10); Fab Fragment | ELISA, WB, IHC, FC | Mouse Fab |
| HPAB-0447-YJ-F(E) | Human Anti-CALR Recombinant Antibody (clone chFMMU-CRT-10); Fab Fragment | ELISA, WB, IHC, FC | Chimeric (mouse/human) Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC841 | AbPlus™ Anti-CALR Magnetic Beads (VS-0724-YC841) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY297 | Anti-CALR Immunohistochemistry Kit | IHC | |
| VS-0525-XY946 | Anti-Mouse CALR Immunohistochemistry Kit | IHC | |
| VS-0525-XY947 | Anti-Human CALR Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC114 | CytoStream™ Rabbit Anti-CALR Recombinant Antibody (VS13-YC114) | WB, ICC, IF, IHC-P, FC, IP | Rabbit IgG |
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