Mouse Anti-NHP CALR Recombinant Antibody (clone FMC 75) (CAT#: VS-1024-XY61)
Employing a mouse-derived monoclonal antibody, this reagent is meticulously designed to target CALR (Calreticulin), exhibiting reactivity with hamster, human, and non-human primate specimens. The immunogen is a calreticulin-maltose binding fusion protein, enhancing specificity and binding affinity. Validated for Western blot (WB) and flow cytometry (FC) applications, this antibody offers a reliable tool for probing CALR expression and localization. Its broad reactivity and robust performance ensure its efficacy across various experimental conditions and species.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
(Immunohistochemical staining of human duodenum shows strong cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/19952/ihc_selected.jpg
Figure 2 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to Endoplasmic reticulum.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/79775/1900_C3_3_blue_red_green.jpg
Figure 3 Cerebral cortex
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
(Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_7_5.jpg
Figure 4 Thyroid gland
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
(Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/2242/8396_B_1_5.jpg
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
Figure 5 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000179218-CALR
Specifications
- Immunogen
- Calreticulin-maltose binding fusion protein
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CALR
- Species Reactivity
- Hamster, Human, Non-human primate
- Clone
- FMC 75
- Applications
- WB, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 0.1 mM PMSF, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:800-1:20, FC: 1:100
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
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| BRD-0076MZ | Chicken Anti-Calreticulin Polyclonal IgY | IHC | Chicken antibody |
| BRD-0772MZ | Chicken Anti-Calreticulin Polyclonal IgY | Blocking Assay (BLOCK) IHC (Paraffin) (IHC (P)) ICC (ICC) Flow Cytometry (Flow) IHC (IHC) | Chicken antibody |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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