Mouse Anti-NHP CDK5 Recombinant Antibody (clone 4E4) (CAT#: VS-1024-XY118)
The mouse-derived monoclonal antibody targets CDK5 and is effective across human, mouse, non-human primate, and rat specimens. The antibody is raised against a purified recombinant fragment of human CDK5, ensuring exceptional specificity and affinity. Suitable for a range of applications including Western blot (WB), immunohistochemistry (IHC), immunofluorescence (IF), flow cytometry (FC), and ELISA, it is an invaluable tool for comprehensive CDK5 research. Its broad reactivity and versatile application profile make it an essential asset for detailed molecular studies.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line SH-SY5Y
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64535/1202_C5_1_selected.jpg
(Immunofluorescent staining of human cell line SH-SY5Y shows localization to nucleoplasm & plasma membrane.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/64535/1202_C5_1_selected.jpg
Figure 2 Testis
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_6_6.jpg
(Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_6_6.jpg
Figure 3 Lymph node
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_8_8.jpg
(Non-germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_8_8.jpg
Figure 4 Cerebral cortex
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_B_8_5.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear Neuropil Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_B_8_5.jpg
Figure 5 Caudate
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_B_9_7.jpg
(Glial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_B_9_7.jpg
Figure 6 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_7_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/8909/22984_A_7_3.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164885-CDK5
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000164885-CDK5
Specifications
- Immunogen
- Purified recombinant fragment of human CDK5
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- CDK5
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 4E4
- Applications
- WB, IHC, IF, FC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, IF: 1:100, FC: 1:200-1:400, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Rabbit Monoclonal Antibody Products
IgG Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-0631 | Hi-Affi™ Rabbit Anti-CDK5 Recombinant Antibody (clone DS631AB) | IHC-P, WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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