Mouse Anti-NHP HSD3B1 Recombinant Antibody (clone FDO66Q)
CAT#: VS-1024-XY259
Designed for exceptional versatility, this mouse monoclonal antibody targets HSD3B1 and shows reactivity across a broad range of species, including baboon, goat, human, mouse, non-human primate, sheep, pig, and rat. The antibody is optimized for immunohistochemistry (IHC) and immunofluorescence (IF), providing reliable and detailed analysis of HSD3B1 expression. Its broad reactivity and robust performance make it an invaluable tool for diverse research applications involving multiple species.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli, endoplasmic reticulum & cytokinetic bridge.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/44028/575_B1_2_selected.jpg
Normal Tissue
Figure 2 Testis
Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43261/160469_A_6_6.jpg
Normal Tissue
Figure 3 Placenta
Trophoblastic cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/43261/160469_A_2_7.jpg
RNA Expression
Figure 4 RNA cell line category: Group enriched (CACO-2, SuSa)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000203857-HSD3B1
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Specifications
- Immunogen
- JEG Choriocarcinoma cells
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- HSD3B1
- Species Reactivity
- Baboon, Goat, Human, Mouse, Non-human primate, Sheep, Pig, Rat
- Clone
- FDO66Q
- Applications
- IHC, IF
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- Tris glycine with 0.15 M NaCl
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 10-20 µg/mL, IF: 10-20 µg/mL
Target
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Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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