Mouse Anti-NHP ITGA2 Recombinant Antibody (clone AK7)
CAT#: VS-1024-XY288
Crafted for the precise detection of ITGA2, this mouse monoclonal antibody demonstrates reactivity with human and non-human primate tissues. It excels in immunohistochemistry (IHC) and flow cytometry (FC), making it an invaluable tool for studying ITGA2 expression and function. With its targeted approach, this antibody supports detailed analysis in both tissue and cell-based assays, ensuring reliable performance across various experimental conditions.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60991/1358_H10_6_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line HaCaT
Immunofluorescent staining of human cell line HaCaT shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/60991/1591_A6_2_red_green.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_B_7_5.jpg
Normal Tissue
Figure 4 Colon
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_A_9_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_A_7_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Collecting ducts
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_A_9_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_A_6_6.jpg
Normal Tissue
Figure 8 Endometrium
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/63556/141951_B_6_3.jpg
RNA Expression
Figure 9 RNA cell line category: Cell line enhanced (BJ hTERT+, HaCaT, hTERT-RPE1, HUVEC TERT2, TIME)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000164171-ITGA2
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Specifications
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- ITGA2
- Species Reactivity
- Human, Non-human primate
- Clone
- AK7
- Applications
- IHC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- IHC: 1:500-1:800, FC: 1:50-1:100
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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| CAT | Product Name | Application | Type |
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| HPAB-0313-CN-S(P) | Human Anti-ITGA2 Recombinant Antibody; scFv Fragment (HPAB-0313-CN-S(P)) | ELISA | Human scFv |
| HPAB-0314-CN-S(P) | Human Anti-ITGA2 Recombinant Antibody; scFv Fragment (HPAB-0314-CN-S(P)) | ELISA | Human scFv |
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| HPAB-0312-CN-F(E) | Mouse Anti-ITGA2 Recombinant Antibody; Fab Fragment (HPAB-0312-CN-F(E)) | ELISA, IHC, Block | Mouse Fab |
| HPAB-0313-CN-F(E) | Human Anti-ITGA2 Recombinant Antibody; Fab Fragment (HPAB-0313-CN-F(E)) | ELISA | Human Fab |
| HPAB-0314-CN-F(E) | Human Anti-ITGA2 Recombinant Antibody; Fab Fragment (HPAB-0314-CN-F(E)) | ELISA | Human Fab |
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|---|---|---|---|
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| CAT | Product Name | Application | Type |
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