Mouse Anti-NHP MLH1 Recombinant Antibody (clone 4C9C7) (CAT#: VS-1024-XY324)
This antibody targets MLH1 and has demonstrated reactivity with human and non-human primate samples. The immunogen comprises a purified recombinant fragment of MLH1, meticulously designed to ensure specificity and reliability. Compatible with a broad array of applications, including Western blot, immunohistochemistry, immunocytochemistry, and ELISA, this antibody provides a versatile tool for researchers investigating DNA mismatch repair pathways.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/if_selected.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/if_selected.jpg
Figure 2 IF staining of human cell line RH-30
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60714/1149_G9_2_selected.jpg
(Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/60714/1149_G9_2_selected.jpg
Figure 3 IHC staining of human tonsil
(Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/ihc_selected.jpg
(Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/ihc_selected.jpg
Figure 4 IHC staining of human gall bladder
(Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13294/ihc_selected.jpg
(Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/13294/ihc_selected.jpg
Figure 5 Kidney
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_7_5.jpg
(Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_7_5.jpg
Figure 6 Testis
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_4_6.jpg
(Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_4_6.jpg
Figure 7 Colon
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_9_3.jpg
(Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_9_3.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076242-MLH1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076242-MLH1
Specifications
- Immunogen
- Purified recombinant fragment of MLH1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MLH1
- Species Reactivity
- Human, Non-human primate
- Clone
- 4C9C7
- Applications
- WB, IHC, ICC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, ELISA: 1:8000
Target
- Alternative Names
- FCC2; COCA2; HNPCC; MLH-1; hMLH1; HNPCC2; LYNCH2; MMRCS1
- Gene ID
- 4292
- UniProt ID
- P40692
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0328MZ | Recombinant Mouse Anti-Human MLH1 Antibody (clone FS06) | IHC | Mouse antibody |
| MRO-1005-CN | Recombinant Rabbit Anti-MLH1 Monoclonal Antibody (CBACN-373) | WB, IF, IHC, IP, FC | Rabbit IgG |
| ZG-0283C | Mouse Anti-MLH1 Recombinant Antibody (ZG-0283C) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0284C | Mouse Anti-MLH1 Recombinant Antibody (ZG-0284C) | WB | Mouse IgG |
| VS3-CJ929 | Mouse Anti-MLH1 Recombinant Antibody (clone ABT-MLH1) | WB, IF, IHC-P | Mouse IgG1, κ |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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