Mouse Anti-NHP MLH1 Recombinant Antibody (clone 4C9C7)
CAT#: VS-1024-XY324
This antibody targets MLH1 and has demonstrated reactivity with human and non-human primate samples. The immunogen comprises a purified recombinant fragment of MLH1, meticulously designed to ensure specificity and reliability. Compatible with a broad array of applications, including Western blot, immunohistochemistry, immunocytochemistry, and ELISA, this antibody provides a versatile tool for researchers investigating DNA mismatch repair pathways.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line RH-30
Immunofluorescent staining of human cell line RH-30 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/60714/1149_G9_2_selected.jpg
Normal Tissue
Figure 3 IHC staining of human tonsil
Immunohistochemical staining of human tonsil shows moderate nuclear positivity in germinal center cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/52707/ihc_selected.jpg
Normal Tissue
Figure 4 IHC staining of human gall bladder
Immunohistochemical staining of human gall bladder shows distinct nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/13294/ihc_selected.jpg
Normal Tissue
Figure 5 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_7_5.jpg
Normal Tissue
Figure 6 Testis
Elongated or late spermatids Staining: Medium Intensity: Moderate Quantity: 75%-25% Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_4_6.jpg
Normal Tissue
Figure 7 Colon
Endothelial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Glandular cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/70868/152351_A_9_3.jpg
RNA Expression
Figure 8 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000076242-MLH1
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Specifications
- Immunogen
- Purified recombinant fragment of MLH1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- MLH1
- Species Reactivity
- Human, Non-human primate
- Clone
- 4C9C7
- Applications
- WB, IHC, ICC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, ELISA: 1:8000
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "MLH1"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-4013z | Mouse Anti-MLH1 Recombinant Antibody (clone 24D7) | WB, IP, IF, ELISA | Mouse IgG2a, κ |
| MOB-0328MZ | Recombinant Mouse Anti-Human MLH1 Antibody (clone FS06) | IHC | Mouse antibody |
| VS3-FY931 | Recombinant Mouse Anti-MLH1 Antibody (clone 2B1C5) | ELISA, WB, IHC, FC | Mouse IgG1 |
| VS3-FY932 | Recombinant Mouse Anti-MLH1 Antibody (clone 5A3F6) | ELISA, FC | Mouse IgG2b |
| VS7-0425-WR391 | Mouse Anti-MLH1 Monoclonal Antibody (VS7-0425-WR391) | WB, IHC-P, IF, ICC, ELISA | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2242 | Hi-Affi™ Recombinant Rabbit Anti-MLH1 Monoclonal Antibody (DS2242AB) | WB, IP, IHC-P, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC731 | CytoStream™ Rabbit Anti-MLH1 Recombinant Antibody (VS13-YC731) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4417 | Anti-MLH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4418 | Anti-Rat MLH1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY4419 | Anti-Mouse MLH1 Immunohistochemistry Kit | IHC |
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