Mouse Anti-NHP PAK2 Recombinant Antibody (clone 3B5) (CAT#: VS-1024-XY363)
This highly specific antibody, derived from mouse, targets PAK2 and is generated using a purified recombinant fragment of the protein as the immunogen. With a remarkable reactivity profile that includes human and non-human primate samples, it proves invaluable for diverse applications such as Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), and ELISA. Designed to deliver precision and reliability, this antibody facilitates the exploration of PAK2's role in cellular signaling and pathology, making it an essential asset for researchers committed to advancing their understanding of complex cellular mechanisms.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & vesicles.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/if_selected.jpg
Figure 2 IHC staining of human colon
(Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/ihc_selected.jpg
(Immunohistochemical staining of human colon shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/ihc_selected.jpg
Figure 3 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_B_8_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_B_8_5.jpg
Figure 4 Colon
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_3.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_3.jpg
Figure 5 Liver
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_4.jpg
(Cholangiocytes Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_4.jpg
Figure 6 Kidney
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_8_5.jpg
(Cells in glomeruli Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_8_5.jpg
Figure 7 Lymph node
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_8.jpg
(Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Non-germinal center cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/7794/25924_A_9_8.jpg
Figure 8 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180370-PAK2
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000180370-PAK2
Specifications
- Immunogen
- Purified recombinant fragment of PAK2
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PAK2
- Species Reactivity
- Human, Non-human primate
- Clone
- 3B5
- Applications
- WB, IHC, ICC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ICC: 1:200-1:800, ELISA: 1:8000
Target
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Bladder Cancer
ErbB Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2750z | Mouse Anti-PAK2 Recombinant Antibody (clone 32A5) | WB, ICC, IF, IHC | Mouse IgG1 |
| MRO-1161-CN | Recombinant Rabbit Anti-PAK2 Monoclonal Antibody (JB41-84) | WB, IF, IHC, FC | Rabbit IgG |
| ZG-0352C | Mouse Anti-PAK2 Recombinant Antibody (ZG-0352C) | WB, IHC, IF, ELISA | Mouse IgG |
| ZG-0496U | Rabbit Anti-PAK2 Recombinant Antibody (clone 6D12) | ELISA, WB, FC, IP | Rabbit IgG |
| VS3-QX849 | Mouse Anti-PAK2 Recombinant Antibody (clone 7H3-E2-F5) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
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For Research Use Only. Not For Clinical Use.
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