Mouse Anti-NHP PCK2 Recombinant Antibody (clone 3F7) (CAT#: VS-1024-XY369)
Targeting PCK2, this mouse-derived antibody is crafted from a human PCK2 recombinant protein, ensuring exceptional specificity and reactivity across human, mouse, non-human primate, and rat samples. Its diverse applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), and Flow Cytometry (FC), make it a versatile tool for exploring metabolic pathways and cellular regulation. With a commitment to precision and reliability, this antibody facilitates in-depth investigations into the role of PCK2 in various physiological and pathological contexts, making it indispensable for cutting-edge research in metabolic biology.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line MCF7
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
(Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
Figure 2 IF staining of human cell line Hep G2
(Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
(Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
Figure 3 Duodenum
(High expression in duodenum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
(High expression in duodenum)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
Figure 4 Small intestine
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
Figure 5 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
Figure 6 Liver
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
(Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, SK-MEL-30)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
Specifications
- Immunogen
- Human PCK2 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- PCK2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 3F7
- Applications
- WB, IHC, ICC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Lyophilized
- Buffer
- PBS with 4 mg trehalose
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.1-0.25 µg/mL, IHC: 2-5 µg/mL, ICC: 5 µg/mL, FC: 1-3 µg/106 cells
Target
- Alternative Names
- Phosphoenolpyruvate Carboxykinase 2; Mitochondrial; EC 4.1.1.32; PEPCK-M; PEPCK2; Phosphoenolpyruvate Carboxykinase [GTP]; Mitochondrial; Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); Phosphopyruvate Carboxylase; PEP Carboxykinase; PEPCK;
- Gene ID
- 5106
- UniProt ID
- Q16822
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
BCR Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2835z | Mouse Anti-PCK2 Recombinant Antibody (clone 34H3) | ELISA, WB | Mouse IgG |
| MOB-1318CT | Recombinant Mouse anti-Human PCK2 Monoclonal antibody (2C9) | ELISA, WB | |
| MRO-1183-CN | Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (JB52-39) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK316 | Rabbit Anti-PCK2 Recombinant Antibody (clone JB52-39) | WB, ELISA | Rabbit IgG |
| VS3-FY1105 | Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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