Mouse Anti-NHP PCK2 Recombinant Antibody (clone 3F7)
CAT#: VS-1024-XY369
Targeting PCK2, this mouse-derived antibody is crafted from a human PCK2 recombinant protein, ensuring exceptional specificity and reactivity across human, mouse, non-human primate, and rat samples. Its diverse applications, including Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), and Flow Cytometry (FC), make it a versatile tool for exploring metabolic pathways and cellular regulation. With a commitment to precision and reliability, this antibody facilitates in-depth investigations into the role of PCK2 in various physiological and pathological contexts, making it indispensable for cutting-edge research in metabolic biology.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
Normal Tissue
Figure 3 Duodenum
High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
Normal Tissue
Figure 4 Small intestine
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
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Specifications
- Immunogen
- Human PCK2 recombinant protein
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- PCK2
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 3F7
- Applications
- WB, IHC, ICC, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Lyophilized
- Buffer
- PBS with 4 mg trehalose
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.1-0.25 µg/mL, IHC: 2-5 µg/mL, ICC: 5 µg/mL, FC: 1-3 µg/106 cells
Target
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Related Signaling Pathways
BCR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Recommended Dilution Buffer
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PCK2"
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2835z | Mouse Anti-PCK2 Recombinant Antibody (clone 34H3) | ELISA, WB | Mouse IgG |
| MOB-1318CT | Recombinant Mouse anti-Human PCK2 Monoclonal antibody (2C9) | ELISA, WB | |
| MRO-1183-CN | Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (JB52-39) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK316 | Rabbit Anti-PCK2 Recombinant Antibody (clone JB52-39) | WB, ELISA | Rabbit IgG |
| VS3-FY1105 | Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2615 | Hi-Affi™ Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (DS2615AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1587 | Anti-PCK2 Immunohistochemistry Kit | IHC |
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