Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6)
CAT#: VS3-FY1105
This product is a recombinant rabbit antibody that recognizes PCK2. This antibody has been reported for use in Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence. The clone R07-8K6 is specific for human, mouse, rat PCK2. The antigen is human pck2 synthetic peptide.
Gene Expression
Tested Data
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
Normal Tissue
Figure 3 Duodenum
High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
Normal Tissue
Figure 4 Small intestine
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
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WB
Figure 1. Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6) in WB.
Western Blot analysis of PCK2 in C6, 3T3, Hela lysates using PCK2 Antibody.
IHC-P
Figure 2. Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6) in IHC-P.
Immunohistochemical analysis of paraffin-embedded human colon carcinoma using a PCK2 antibody. High pressure and high temperature sodium citrate pH 6.0 for antigen retrieval.
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Specifications
- Immunogen
- Human PCK2 Synthetic Peptide.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse, Rat PCK2
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R07-8K6
- Applications
- Western Blot, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunocytochemistry, Immunofluorescence
- Conjugate
- Unconjugated
- MW
- Calculated MW: 71 kDa; Observed MW: 71 kDa
Product Property
- Purification
- Affinity Purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- 50 mM Tris-Glycine, pH 7.4, 0.15 M NaCl, 40% glycerol, 0.05% BSA
- Preservative
- 0.01% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry-Paraffin: 1/50-1/100
Immunofluorescence: 1/50-1/200
Target
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Related Signaling Pathways
BCR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PCK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1318CT | Recombinant Mouse anti-Human PCK2 Monoclonal antibody (2C9) | ELISA, WB | |
| VS3-WK316 | Rabbit Anti-PCK2 Recombinant Antibody (clone JB52-39) | WB, ELISA | Rabbit IgG |
| VS7-0425-WR573 | Mouse Anti-PCK2 Recombinant Antibody (clone 3D3E11) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR574 | Mouse Anti-PCK2 Recombinant Antibody (clone 3D3D9) | WB, IHC, ICC, FC | Mouse IgG1 |
| VS7-0425-WR575 | Mouse Anti-PCK2 Recombinant Antibody (VS7-0425-WR575) | WB | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2615 | Hi-Affi™ Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (DS2615AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY369 | Mouse Anti-NHP PCK2 Recombinant Antibody (clone 3F7) | WB, IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1587 | Anti-PCK2 Immunohistochemistry Kit | IHC |
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