Mouse Anti-NHP RUVBL2 Recombinant Antibody (clone OTI1A6) (CAT#: VS-1024-XY458)
Designed for precision, this monoclonal antibody targets RUVBL2 and demonstrates extensive reactivity across a range of species, including dog, human, mouse, non-human primate, and rat. Utilizing a human recombinant protein fragment as the immunogen, it ensures specificity and effective interaction. Suitable for Western blotting, immunohistochemistry, immunofluorescence, and flow cytometry applications, this antibody is invaluable for researchers investigating RUVBL2's pivotal roles in cellular functions and disease mechanisms. Its adaptability and reliability make it a critical tool in the exploration of RUVBL2-related pathways and their implications in health and disease.
Specific Inquiry
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42880/736_G2_3_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoplasm & cytosol.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/42880/736_G2_3_selected.jpg
Figure 2 IF staining of human cell line A549
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67966/1718_F2_19_cr57f3d591e8263_selected.jpg
(Immunofluorescent staining of human cell line A549 shows localization to nucleoplasm, cytosol & centrosome.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67966/1718_F2_19_cr57f3d591e8263_selected.jpg
Figure 3 Testis
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67966/161986_A_4_6.jpg
(Elongated or late spermatids Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Preleptotene spermatocytes Staining: Medium Intensity: Moderate Quantity:>75% Round or early spermatids Staining: High Intensity: Strong Quantity:>75% Sertoli cells Staining: Low Intensity: Weak Quantity:>75% Spermatogonia cells Staining: Medium Intensity: Moderate)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/67966/161986_A_4_6.jpg
Figure 4 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183207-RUVBL2
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000183207-RUVBL2
Specifications
- Immunogen
- Human recombinant protein fragment
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- RUVBL2
- Species Reactivity
- Dog, Human, Mouse, Non-human primate, Rat
- Clone
- OTI1A6
- Applications
- WB, IHC, IF, FC
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4, with 1% BSA, 50% glycerol
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:20, IHC: 1:200, IF: 1:100, FC: 1:100
Target
- Alternative Names
- RVB2; TIH2; ECP51; TIP48; CGI-46; ECP-51; INO80J; REPTIN; TIP49B; TAP54-beta
- Gene ID
- 10856
- UniProt ID
- Q9Y230
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IgG Antibody Products
Rabbit Monoclonal Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2874z | Mouse Anti-RUVBL2 Recombinant Antibody (clone 6B6) | WB, FC, IF, IHC | Mouse IgG1 |
| VS3-XY3492 | Rabbit Anti-RUVBL2 Recombinant Antibody (clone R05-8F9) | WB | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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