Rabbit Anti-NONO Recombinant Antibody (clone 8C11) (CAT#: ZG-0061U)
The purified Rabbit Anti-NONO Recombinant Antibody (clone 8C11) displayed high specificity against the NONO protein of human.
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Custom Production
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WB
Figure 1 Rabbit Anti-NONO Antibody (ZG-0061U) in WB
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate. All lanes: NONO Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44 kDa
Observed band size: 55 kDa
IHC
Figure 2 Rabbit Anti-NONO Antibody (ZG-0061U) in IHC
IHC image of ZG-0061U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-NONO Antibody (ZG-0061U) in IF
Immunofluorescence staining of Hela Cells with ZG-0061U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
Specifications
- Immunogen
- A synthesized peptide derived from human NONO / p54nrb
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NONO
- Species Reactivity
- Human
- Clone
- 8C11
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Intellectual Developmental Disorder, X-Linked, Syndromic 34 and Renal Cell Carcinoma, Xp11-Associated
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
- Alternative Names
- Non-POU domain-containing octamer-binding protein; NonO protein; 54 kDa nuclear RNA- and DNA-binding protein; 55 kDa nuclear protein; DNA-binding p52/p100 complex; 52 kDa subunit; NMT55; p54; nrb; p54nrb; NONO; NRB54
- Gene ID
- 4841
- UniProt ID
- Q15233
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-3007z | Mouse Anti-NONO Recombinant Antibody (clone 24H3) | WB, IF, IHC | Mouse IgG1 |
| HPAB-0766-CN | Human Anti-NONO Recombinant Antibody (HPAB-0766-CN) | WB, ELISA, IHC, IP | Human IgG |
| MRO-1154-CN | Recombinant Rabbit Anti-NONO Monoclonal Antibody (JG87-38) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1048 | Recombinant Rabbit Anti-NONO Antibody (clone R05-1F8) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS13-YC824 | Rabbit Anti-NONO Recombinant Antibody (VS13-YC824) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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