Rabbit Anti-NONO Recombinant Antibody (clone 8C11)
CAT#: ZG-0061U
The purified Rabbit Anti-NONO Recombinant Antibody (clone 8C11) displayed high specificity against the NONO protein of human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-NONO Antibody (ZG-0061U) in WB
Positive WB detected in: 293 whole cell lysate, Hela whole cell lysate, HepG2 whole cell lysate, Ntera-2 whole cell lysate. All lanes: NONO Antibody at 1: 1000
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 55, 44 kDa
Observed band size: 55 kDa
IHC
Figure 2 Rabbit Anti-NONO Antibody (ZG-0061U) in IHC
IHC image of ZG-0061U diluted at 1:100 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
IF
Figure 3 Rabbit Anti-NONO Antibody (ZG-0061U) in IF
Immunofluorescence staining of Hela Cells with ZG-0061U at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).
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Subcellular Location
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/877_A7_2_selected.jpg
Normal Tissue
Figure 2 IHC staining of human skeletal muscle
Immunohistochemical staining of human skeletal muscle shows strong nuclear positivity and additional strong cytoplasmic staining.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/22069/ihc_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Endothelial cells Staining: High Intensity: Strong Quantity: 75%-25% Location: Nuclear Glial cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_B_8_5.jpg
Normal Tissue
Figure 4 Colon
Endocrine cells Staining: High Intensity: Strong Endothelial cells Staining: High Intensity: Strong Quantity:>75% Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: Medium Intensity: Moderate Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: High Intensity: Strong Quantity:>75% Peripheral nerve/ganglion Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_3.jpg
Normal Tissue
Figure 5 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: nuclear Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_4.jpg
Normal Tissue
Figure 6 Kidney
Cells in glomeruli Staining: High Intensity: Strong Quantity:>75% Location: Nuclear Cells in tubules Staining: High Intensity: Strong Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_5.jpg
Normal Tissue
Figure 7 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75% Pachytene spermatocytes Staining: High Intensity: Strong Quantity: 75%-25% Peritubular cells Staining: High Intensity: Strong Quantity:>75% Preleptotene spermatocytes Staining: High Intensity: Strong Quantity:>75% Round or early spermatids Staining: Low Intensity: Weak Quantity:>75% Sertoli cells Staining: High Intensity: Strong Quantity:>75% Spermatogonia cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_6_6.jpg
Normal Tissue
Figure 8 Ovary
Follicle cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear Ovarian stroma cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_5_7.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/54094/139334_A_8_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000147140-NONO
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Specifications
- Immunogen
- A synthesized peptide derived from human NONO / p54nrb
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human NONO
- Species Reactivity
- Human
- Clone
- 8C11
- Applications
- ELISA, WB, IHC, IF
- Conjugate
- Unconjugated
- Related Disease
- Intellectual Developmental Disorder, X-Linked, Syndromic 34 and Renal Cell Carcinoma, Xp11-Associated
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, western blot, Immunohistochemistry, Immunofluorescence. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200, IF:1:20-1:200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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|---|---|---|---|
| MOB-3007z | Mouse Anti-NONO Recombinant Antibody (clone 24H3) | WB, IF, IHC | Mouse IgG1 |
| HPAB-0766-CN | Human Anti-NONO Recombinant Antibody (HPAB-0766-CN) | WB, ELISA, IHC, IP | Human IgG |
| MRO-1154-CN | Recombinant Rabbit Anti-NONO Monoclonal Antibody (JG87-38) | WB, IF, IHC | Rabbit IgG |
| VS3-FY1048 | Recombinant Rabbit Anti-NONO Antibody (clone R05-1F8) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS13-YC824 | Rabbit Anti-NONO Recombinant Antibody (VS13-YC824) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ | Anti-Human NONO Recombinant Antibody (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| TAB-538MZ-S(P) | Anti-Human NONO Recombinant Antibody scFv Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| TAB-538MZ-F(E) | Anti-Human NONO Recombinant Antibody Fab Fragment (PAT-LM1) | WB, SDS-PAGE, ELISA | Human antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2467 | Hi-Affi™ Recombinant Rabbit Anti-NONO Monoclonal Antibody (DS2467AB) | FC, ICC, IHC-P, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-S(P) | Human Anti-NONO Recombinant Antibody; scFv Fragment (HPAB-0766-CN-S(P)) | WB, ELISA, IHC, IP | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0766-CN-F(E) | Human Anti-NONO Recombinant Antibody; Fab Fragment (HPAB-0766-CN-F(E)) | WB, ELISA, IHC, IP | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY4894 | Anti-NONO Immunohistochemistry Kit | IHC | |
| VS-0525-XY4895 | Anti-Mouse NONO Immunohistochemistry Kit | IHC |
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