Mouse Anti-NPM1 Monoclonal Antibody (VS7-0425-WR495)
CAT#: VS7-0425-WR495
This product is a mouse IgG antibody that binds to human NPM1. The antibody can be used for WB, IHC-P, IF, ICC, and ELISA assays. It cross reacts with mouse.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm & nucleoli rim.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/108_B11_2_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human colon
Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human cerebellum
Immunohistochemical staining of human cerebellum shows strong nuclear positivity in cerebellar tissue.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/ihc_selected.jpg
Normal Tissue
Figure 4 Cerebral cortex
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_B_7_5.jpg
Normal Tissue
Figure 5 Colon
Endothelial cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Glandular cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Cholangiocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Hepatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/11384/28259_A_8_4.jpg
Normal Tissue
Figure 7 Kidney
Cells in glomeruli
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Cells in tubules
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_8_5.jpg
Normal Tissue
Figure 8 Testis
Pachytene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Peritubular cells
Staining: High
Intensity: Strong
Quantity:>75%
Preleptotene spermatocytes
Staining: High
Intensity: Strong
Quantity:>75%
Round or early spermatids
Staining: High
Intensity: Strong
Quantity:>75%
Sertoli cells
Staining: High
Intensity: Strong
Quantity:>75%
Spermatogonia cells
Staining: High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_6_6.jpg
Normal Tissue
Figure 9 Lymph node
Germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12983/28235_A_7_8.jpg
RNA Expression
Figure 10 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000181163-NPM1
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Specifications
- Immunogen
- Recombinant human NPM1 fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human NPM1
- Species Reactivity
- Human, Monkey
- Molecule Class
- Phosphoprotein
- Applications
- WB, IHC-P, IF, ICC, ELISA
- Conjugate
- Unconjugated
- Related Disease
- A chromosomal aberration involving NPM1 is found in a form of non-Hodgkin lymphoma. Translocation t(2;5)(p23;q35) with ALK. The resulting chimeric NPM1-ALK protein homodimerize and the kinase becomes constitutively activated2 publications
A chromosomal aberration involving NPM1 is found in a form of acute promyelocytic leukemia. Translocation t(5;17)(q32;q11) with RARA1 publication
A chromosomal aberration involving NPM1 is a cause of myelodysplastic syndrome (MDS). Translocation t(3;5)(q25.1;q34) with MLF11 publication
Defects in NPM1 are associated with acute myelogenous leukemia (AML). Mutations in exon 12 affecting the C-terminus of the protein are associated with an aberrant cytoplasmic location
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunofluorescence: 1:200 - 1:1000
Immunohistochemistry: 1:200 - 1:1000
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- NPM1; nucleophosmin (nucleolar phosphoprotein B23, numatrin); B23; NPM; nucleophosmin; nucleolar protein NO38; nucleophosmin/nucleoplasmin family, member 1
- Gene ID
- 4869
- UniProt ID
- P06748
- Long Name
- Nucleophosmin 1
- Cellular Localization
- Cytoplasm; Cytoskeleton; Nucleus
- Post Translation Modifications
- Acetylation; ADP-ribosylation; Disulfide bond; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001032827.1
- Function
- Chaperone; RNA-binding
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
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