Mouse Anti-P4HB Recombinant Antibody (clone 4E10C6)
CAT#: VS7-0425-WR551
This product is a mouse IgG2a antibody that binds to human P4HB. The antibody 4E10C6 recognizes specific amino acid domain of human P4HB (AA:309-508). It can be used for WB, IHC, and FC assays.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/if_selected.jpg
Normal Tissue
Figure 2 Small intestine
High expression in small intestine
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_2_val_selected.jpg
Normal Tissue
Figure 3 Testis
Leydig cells Staining: High Intensity: Strong Quantity:>75%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_6.jpg
Normal Tissue
Figure 4 Duodenum
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Glands of Brunner Staining: High Intensity: Strong Quantity:>75% Goblet cells Staining: High Intensity: Strong Quantity:>75% Paneth cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_9_2.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_4.jpg
RNA Expression
Figure 7 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185624-P4HB
❮
❯
Specifications
- Immunogen
- Recombinant human P4HB (AA:309-508) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human P4HB
- Species Reactivity
- Human
- Clone
- 4E10C6
- Molecule Class
- Polyhydroxyalkanoates (PHAS)
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cole-Carpenter syndrome 1 (CLCRP1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
- Gene ID
- 5034
- UniProt ID
- P07237
- Long Name
- Prolyl 4-Hydroxylase Subunit Beta
- Cellular Localization
- Cell membrane; Endoplasmic reticulum; Membrane
- Post Translation Modifications
- Acetylation; Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_000909.2
- Function
- Chaperone; Isomerase
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS7-0425-WR551. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
Reconstitution Buffer
- CAT
- Product Name
Recommended Dilution Buffer
- CAT
- Product Name
See other products for "P4HB"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2363CT | Recombinant Mouse anti-Cow P4HB Monoclonal antibody (nBcdbn72940) | FC | |
| VS3-FY1086 | Recombinant Mouse Anti-P4HB Antibody (clone 3F12C11D2) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1087 | Recombinant Mouse Anti-P4HB Antibody (clone 5D12A4E5) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS3-FY1088 | Recombinant Rabbit Anti-P4HB Antibody (clone R07-3H3) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR550 | Mouse Anti-P4HB Recombinant Antibody (clone 4E10C6F6) | WB, IHC, ICC, FC | Mouse IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1823CQ | Recombinant Mouse Anti-P4hb Antibody (RL77) | BL, ELISA, FC, GSA, ICC, IF, IHC, IHC-P, IP, WB | IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| NEUT-1824CQ | Recombinant Mouse Anti-P4hb Antibody (RL90) | BL, ELISA, FC, ICC, IF, IHC, IHC-Fr, IHC-P, IP, WB | IgG2a |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2568 | Hi-Affi™ Recombinant Rabbit Anti-P4HB Monoclonal Antibody (DS2568AB) | WB, IHC, ICC, FC | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0724-YC1441 | AbPlus™ Anti-P4HB Magnetic Beads (VS-0724-YC1441) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5140 | Anti-Mouse P4HB Immunohistochemistry Kit | IHC | |
| VS-0525-XY5141 | Anti-Rabbit P4HB Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IP, ELISA, Neut, FuncS, IF, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: WB, FuncS, IF, Neut, ELISA, FC, IP
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: FC, IHC, FuncS, Inhib, Cyt
Application: IP, IF, FuncS, FC, Neut, ELISA, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: ELISA
Application: WB, ELISA, FuncS, IB, FC, SPR, Apop
Application: ELISA, IHC, FC, IP, IF, FuncS
Application: ELISA
Application: ELISA, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
