Mouse Anti-P4HB Recombinant Antibody (clone 4E10C6) (CAT#: VS7-0425-WR551)
This product is a mouse IgG2a antibody that binds to human P4HB. The antibody 4E10C6 recognizes specific amino acid domain of human P4HB (AA:309-508). It can be used for WB, IHC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to endoplasmic reticulum.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/if_selected.jpg
Figure 2 Small intestine
(High expression in small intestine)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_2_val_selected.jpg
(High expression in small intestine)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_2_val_selected.jpg
Figure 3 Testis
(Leydig cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_6.jpg
(Leydig cells Staining: High Intensity: Strong Quantity:>75%)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_4_6.jpg
Figure 4 Duodenum
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Glands of Brunner Staining: High Intensity: Strong Quantity:>75% Goblet cells Staining: High Intensity: Strong Quantity:>75% Paneth cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_9_2.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Glands of Brunner Staining: High Intensity: Strong Quantity:>75% Goblet cells Staining: High Intensity: Strong Quantity:>75% Paneth cells Staining: High Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_9_2.jpg
Figure 5 Colon
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_3.jpg
(Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Fibroblasts Staining: High Intensity: Strong Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Strong)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_3.jpg
Figure 6 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/18884/43373_A_7_4.jpg
Figure 7 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185624-P4HB
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000185624-P4HB
Specifications
- Immunogen
- Recombinant human P4HB (AA:309-508) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human P4HB
- Species Reactivity
- Human
- Clone
- 4E10C6
- Molecule Class
- Polyhydroxyalkanoates (PHAS)
- Applications
- WB, IHC, FC
- Conjugate
- Unconjugated
- Related Disease
- Cole-Carpenter syndrome 1 (CLCRP1)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS
- Preservative
- 0.05% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- DSI; GIT; PDI; PHDB; PDIA1; PO4DB; PO4HB; PROHB; CLCRP1; ERBA2L; P4Hbeta
- Gene ID
- 5034
- UniProt ID
- P07237
- Long Name
- Prolyl 4-Hydroxylase Subunit Beta
- Cellular Localization
- Cell membrane; Endoplasmic reticulum; Membrane
- Post Translation Modifications
- Acetylation; Disulfide bond; Phosphoprotein
- Protein Refseq
- NP_000909.2
- Function
- Chaperone; Isomerase
Recommended Products
Isotype Control
Secondary Antibody
Reconstitution Buffer
Recommended Dilution Buffer
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Antibody Magnetic Beads
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1426z | Mouse Anti-P4HB Recombinant Antibody (clone 31F5) | WB, IP, IF, IHC, ELISA | Mouse IgG1, κ |
| MOB-2363CT | Recombinant Mouse anti-Cow P4HB Monoclonal antibody (nBcdbn72940) | FC | |
| VS3-CJ460 | Mouse Anti-P4HB Recombinant Antibody (VS3-CJ460) | WB | Mouse IgG |
| VS3-FY1086 | Recombinant Mouse Anti-P4HB Antibody (clone 3F12C11D2) | ELISA, WB, IHC, ICC, FC | Mouse IgG2a |
| VS7-0425-WR550 | Mouse Anti-P4HB Recombinant Antibody (clone 4E10C6F6) | WB, IHC, ICC, FC | Mouse IgG2a |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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