Mouse Anti-PBK Monoclonal Antibody (clone 2C8) (CAT#: VS7-0425-WR570)
This product is a mouse IgG2b antibody that binds to human PBK. The antibody 2C8 can be used for WB, IHC, ICC, and FC assays.
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
We require custom production
Subcellular Location and Protein Expression
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line U-2 OS
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
(Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Figure 2 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Figure 3 IF staining of human cell line U-251 MG
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
(Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Figure 4 Testis
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
(Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
Figure 5 RNA cell line category: Cell line enhanced (A-431)
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
Specifications
- Immunogen
- Recombinant human PBK fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human PBK
- Species Reactivity
- Human
- Clone
- 2C8
- Molecule Class
- Serine/Threonine Protein Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PBK; PDZ binding kinase; SPK; CT84; TOPK; HEL164; Nori-3; lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; T-LAK cell-originated protein kinase; cancer/testis antigen 84; epididymis luminal protein 164; serine/threo
- Gene ID
- 55872
- UniProt ID
- Q96KB5
- Long Name
- PDZ Binding Kinase
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001265874.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
Recommended Products
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2606 | Hi-Affi™ Recombinant Rabbit Anti-PBK Monoclonal Antibody (DS2606AB) | IP, WB | IgG |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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