Mouse Anti-PBK Monoclonal Antibody (clone 2C8)
CAT#: VS7-0425-WR570
This product is a mouse IgG2b antibody that binds to human PBK. The antibody 2C8 can be used for WB, IHC, ICC, and FC assays.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/11_H7_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/1899_B5_1_cr5ba20dcb55d2a_blue_red_green.jpg
Normal Tissue
Figure 4 Testis
Cells in seminiferous ducts
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/5753/159879_A_5_6.jpg
RNA Expression
Figure 5 RNA cell line category: Cell line enhanced (A-431)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000168078-PBK
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Specifications
- Immunogen
- Recombinant human PBK fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG2b
- Specificity
- Human PBK
- Species Reactivity
- Human
- Clone
- 2C8
- Molecule Class
- Serine/Threonine Protein Kinase
- Applications
- WB, IHC, ICC, FC
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Preservative
- 0.03% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:500 - 1:2000
Immunohistochemistry-Paraffin: 1:200 - 1:1000
Immunocytochemistry: 1:200 - 1:1000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000
Target
- Alternative Names
- PBK; PDZ binding kinase; SPK; CT84; TOPK; HEL164; Nori-3; lymphokine-activated killer T-cell-originated protein kinase; MAPKK-like protein kinase; T-LAK cell-originated protein kinase; cancer/testis antigen 84; epididymis luminal protein 164; serine/threo
- Gene ID
- 55872
- UniProt ID
- Q96KB5
- Long Name
- PDZ Binding Kinase
- Cellular Localization
- Nucleus
- Post Translation Modifications
- Acetylation; Isopeptide bond; Phosphoprotein; Ubl conjugation
- Protein Refseq
- NP_001265874.1
- Function
- Kinase; Serine/threonine-protein kinase; Transferase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PBK"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| DrMAB-427 | Recombinant Human Anti-Human PBK Antibody, clone D532 | ELISA, IP-MS | IgG |
| VS3-QX851 | Mouse Anti-PBK Recombinant Antibody (clone 4F8-C6-A10) | WB, IHC, ICC, FC, ELISA | Mouse IgG2b |
| VS3-WK24 | Mouse Anti-PBK Recombinant Antibody (clone E11-C8) | WB, ELISA | Mouse IgG2b |
| VS3-FY1816 | Rabbit Anti-PBK Recombinant Antibody (clone R05-1B6) | WB, IHC-P, IP | Rabbit IgG |
| VS13-YC872 | Rabbit Anti-PBK Recombinant Antibody (VS13-YC872) | IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2606 | Hi-Affi™ Recombinant Rabbit Anti-PBK Monoclonal Antibody (DS2606AB) | IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1572 | Anti-PBK Immunohistochemistry Kit | IHC | |
| VS-0525-XY5218 | Anti-Mouse PBK Immunohistochemistry Kit | IHC | |
| VS-0525-XY5217 | Anti-Human PBK Immunohistochemistry Kit | IHC |
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