Mouse Anti-PCK2 Recombinant Antibody (VS7-0425-WR575)
CAT#: VS7-0425-WR575
This product is a mouse IgG antibody that binds to human PCK2. The antibody can be used for WB assay. It cross reacts with mouse, rat, bovine, dog, and pig.
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/if_selected.jpg
Subcellular Location
Figure 2 IF staining of human cell line Hep G2
Immunofluorescent staining of human cell line Hep G2 shows localization to mitochondria.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/53502/1334_B5_2_selected.jpg
Normal Tissue
Figure 3 Duodenum
High expression in duodenum
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_7_2_selected.jpg
Normal Tissue
Figure 4 Small intestine
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Gradient Gradient: Ascending Enterocytes - Goblet cells Staining: High Intensity: Strong
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_4_2.jpg
Normal Tissue
Figure 5 Colon
Endocrine cells Staining: High Intensity: Strong Enterocytes Staining: High Intensity: Strong Quantity:>75% Enterocytes - Goblet cells Staining: High Intensity: Strong Quantity:>75% Mucosal lymphoid cells Staining: Medium Intensity: Moderate Quantity: 75%-25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_3.jpg
Normal Tissue
Figure 6 Liver
Hepatocytes Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/51162/124859_A_9_4.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (HHSteC, hTCEpi, SK-MEL-30)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000100889-PCK2
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Specifications
- Immunogen
- Recombinant human PCK2 (C-terminus) fragment expressed in E. Coli.
- Host Species
- Mouse
- Type
- Mouse IgG
- Specificity
- Human PCK2
- Species Reactivity
- Human, Mouse, Rat, Bovine, Dog, Pig
- Molecule Class
- Phosphoenolpyruvate Carboxykinase
- Applications
- WB
- Conjugate
- Unconjugated
- Related Disease
- Mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD)
Product Property
- Clonality
- Monoclonal
- Purity
- >95% as determined by SDS-PAGE and SEC-HPLC analysis, Ascitic fluid
- Format
- Liquid
- Concentration
- 1 mg/ml
- Buffer
- 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl)
- Preservative
- 0.2% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Aliquot and store at -20°C for one year. Avoid repeated freeze/thaw cycles.
- Shipping
- Ice packs
Applications
- Application Notes
- Western Blot: 1:1000 - 1:2000
Target
- Alternative Names
- PCK2; Phosphoenolpyruvate Carboxykinase [GTP], Mitochondrial; PEPCK; PEPCK-M; PE; PEP Carboxykinase; EC 4.1.1.32; Phosphoenolpyruvate Carboxykinase 2 (Mitochondrial); Phosphopyruvate Carboxylase
- Gene ID
- 5106
- UniProt ID
- Q16822
- Long Name
- Phosphoenolpyruvate Carboxykinase 2, Mitochondrial
- Cellular Localization
- Mitochondrion
- Post Translation Modifications
- Acetylation; Phosphoprotein
- Protein Refseq
- NP_001018083.2
- Function
- Decarboxylase; Lyase
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Related Signaling Pathways
BCR Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PCK2"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2615 | Hi-Affi™ Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (DS2615AB) | WB, IHC-P | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-1183-CN | Recombinant Rabbit Anti-PCK2 Monoclonal Antibody (JB52-39) | WB, IF, IHC, FC | Rabbit IgG |
| VS3-WK316 | Rabbit Anti-PCK2 Recombinant Antibody (clone JB52-39) | WB, ELISA | Rabbit IgG |
| VS3-FY1105 | Recombinant Rabbit Anti-PCK2 Antibody (clone R07-8K6) | WB, IHC-Fr, IHC-P, ICC, IF | Rabbit IgG |
| VS7-0425-WR573 | Mouse Anti-PCK2 Recombinant Antibody (clone 3D3E11) | WB, IHC, FC | Mouse IgG1 |
| VS7-0425-WR574 | Mouse Anti-PCK2 Recombinant Antibody (clone 3D3D9) | WB, IHC, ICC, FC | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY369 | Mouse Anti-NHP PCK2 Recombinant Antibody (clone 3F7) | WB, IHC, ICC, FC | Mouse IgG2b |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1587 | Anti-PCK2 Immunohistochemistry Kit | IHC |
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