This product is a recombinant Mouse antibody that recognizes PCNA. The antibody was expressed in mammalian cells with chemically defined culture media and was purified by affinity chromatography.
Figure 1 Anti-PCNA Recombinant Antibody (HPAB-N0376-YC) in WB
Western blot analysis of HPAB-N0376-YC was performed with Human PCNA Protein (His Tag) in reduced condition Lane 1, Lane 2 and Lane 3 (0.1 μg, 0.3 μg and 0.6 μg respectively) onto a 12% Tris-HCl polyacrylamide gel. Proteins were transferred to a CN membrane and blocked with 5% skim milk for at least one hour. Membranes were probed with HPAB-N0376-YC (2 μg/mL) and HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:6000). Chemiluminescent detection was performed.
Figure 2 Anti-PCNA Recombinant Antibody (HPAB-N0376-YC) in DB
Dot Blot analysis of HPAB-N0376-YC was performed by coating with Human PCNA Protein (His Tag).
HPAB-N0376-YC incubation concentration: 2 μg/mL.
HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:6000)
Figure 3 Anti-PCNA Recombinant Antibody (HPAB-N0376-YC) in ELISA
ELISA analysis of HPAB-N0376-YC was performed by coating with Human PCNA Protein (His Tag). Then blocked with BSA and incubated with HPAB-N0376-YC. The HRP-conjugated goat anti-mouse IgG as a secondary antibody (1:6000). Detection was performed using TMB substrate and stopped with sulfuric acid. The absorbances were read on a spectrophotometer at 450 nm.
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Download resources about recombinant antibody development and antibody engineering to boost your research.
CAT | Product Name | Application | Type |
---|---|---|---|
MOR-2616 | Hi-Affi™ Recombinant Rabbit Anti-PCNA Monoclonal Antibody (DS2616AB) | FC, IHC-P, WB | IgG |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0205-YC | Mouse Anti-PCNA Recombinant Antibody (clone 12D10) | ELISA, WB, IHC | Mouse IgG |
MOB-0220F | Mouse Anti-PCNA Recombinant Antibody (MOB-0220F) | IHC-P, IF | Mouse IgG |
MOB-0221F | Mouse Anti-PCNA Recombinant Antibody (MOB-0221F) | IHC-P, IF | Mouse IgG |
VS3-CJ608 | Mouse Anti-PCNA Recombinant Antibody (clone ABT-PCNA) | WB, IHC-P | Mouse IgG1, κ |
VS3-CJ609 | Mouse Anti-PCNA Recombinant Antibody (clone PT0221) | WB, IHC-P | Mouse IgG1, κ |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0205-YC-S(P) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); scFv Fragment | ELISA, WB, IHC | Mouse scFv |
HPAB-N0376-YC-S(P) | Mouse Anti-PCNA Recombinant Antibody; scFv Fragment (HPAB-N0376-YC-S(P)) | ELISA, FC, WB | Mouse scFv |
CAT | Product Name | Application | Type |
---|---|---|---|
HPAB-0205-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody (clone 12D10); Fab Fragment | ELISA, WB, IHC | Mouse Fab |
HPAB-N0376-YC-F(E) | Mouse Anti-PCNA Recombinant Antibody; Fab Fragment (HPAB-N0376-YC-F(E)) | ELISA, FC, WB | Mouse Fab |
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For Research Use Only. Not For Clinical Use.
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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