Mouse Anti-NHP PCNA Recombinant Antibody (clone PC10) (CAT#: VS-1024-XY370)
Designed for the precise detection of PCNA, this mouse monoclonal antibody demonstrates reactivity with human, insect, mouse, non-human primate, and rat tissues. The immunogen consists of rat recombinant PCNA polypeptides, ensuring exceptional specificity. Versatile in application, it is suitable for Western blot (WB), immunohistochemistry (IHC), immunocytochemistry (ICC), and immunoprecipitation (IP), making it an indispensable tool for studying PCNA expression and function across a variety of biological contexts.
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We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
Figure 2 Colon
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
Figure 3 Testis
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
Figure 4 Bone marrow
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
Figure 5 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
Specifications
- Immunogen
- Rat recombinant PCNA polypeptides
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- PCNA
- Species Reactivity
- Human, Insect, Mouse, Non-human primate, Rat
- Clone
- PC10
- Applications
- WB, IHC, ICC, IP
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 0.1-1 µg/mL, IHC: 1:50-1:500, ICC: 1-2 µg/mL, IP: 2-5 µg
Target
- Alternative Names
- Proliferating Cell Nuclear Antigen; Cyclin; DNA Polymerase Delta Auxiliary Protein; ATLD2;
- Gene ID
- 5111
- UniProt ID
- P12004
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2616 | Hi-Affi™ Recombinant Rabbit Anti-PCNA Monoclonal Antibody (DS2616AB) | FC, IHC-P, WB | IgG |
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