CytoStream™ Rabbit Anti-PCNA Recombinant Antibody (VS13-YC876) (CAT#: VS13-YC876)
The CytoStream™ Rabbit Anti-PCNA Recombinant Antibody is specific for human and mouse PCNA. PCNA encodes a replication cofactor for DNA polymerase delta, facilitating DNA repair and synthesis, with ubiquitinated involvement in DNA repair pathways and pseudogenes mapped to chromosomes 4 and X. The anti-PCNA antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
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Custom Production
We specialize in custom recombinant antibody production, offering seamless execution from provided sequences to high-quality antibody deliverables, ensuring optimal yield and purity.
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Subcellular Location
Normal Tissue
RNA Expression
Figure 1 IF staining of human cell line A-431
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
(Immunofluorescent staining of human cell line A-431 shows localization to nucleoplasm.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/148/if_selected.jpg
Figure 2 Colon
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
(Glandular cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_9_3.jpg
Figure 3 Testis
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
(Leydig cells
Staining:
Medium
Intensity: Moderate
Quantity: 75%-25%
Preleptotene spermatocytes
Staining:
Medium
Intensity: Strong
Quantity: <25%)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_6_6.jpg
Figure 4 Bone marrow
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
(Hematopoietic cells
Staining:
High
Intensity: Strong
Quantity: 75%-25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_B_5_4.jpg
Figure 5 Lymph node
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
(Germinal center cells
Staining:
High
Intensity: Strong
Quantity:>75%
Location: Nuclear
Non-germinal center cells
Staining:
Medium
Intensity: Strong
Quantity: <25%
Location: Nuclear)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/30523/64162_A_7_8.jpg
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
Figure 6 RNA cell line category: Low cell line specificity
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
(Cell lines ordered by descending RNA expression order.)
* Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000132646-PCNA
Specifications
- Immunogen
- Recombinant human PCNA, aa 88-137.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human and Mouse PCNA
- Species Reactivity
- Human, Mouse
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
- Related Disease
- Ataxia-Telangiectasia-Like Disorder and Bowen's Disease
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/5000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/100-1/500
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Proliferating Cell Nuclear Antigen; Cyclin; DNA Polymerase Delta Auxiliary Protein; ATLD2;
- Gene ID
- 5111
- UniProt ID
- P12004
- Long Name
- Proliferating Cell Nuclear Antigen
- Sequence Similarities
- Belongs to the PCNA family.
- Cellular Localization
- Nucleus
- Protein Refseq
- NP_002583.1; NP_872590.1
- Function
- Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion.
Recommended Products
Secondary Antibody
Isotype Control
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
Download resources about recombinant antibody development and antibody engineering to boost your research.
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IHC Kit and Antibody Products: Precision Tools for Immunohistochemistry
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2616 | Hi-Affi™ Recombinant Rabbit Anti-PCNA Monoclonal Antibody (DS2616AB) | FC, IHC-P, WB | IgG |
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