Mouse Anti-PDE5A Recombinant Antibody (PD134)
CAT#: VS-0326-YC134
The anti-PDE5A recombinant antibody (PD134) reacts with human PDE5A. PDE5A is a cGMP-specific phosphodiesterase that regulates the concentration of intracellular cGMP by catalyzing its degradation to 5'-GMP. It is primarily found in vascular smooth muscle and the corpus cavernosum. PDE5A signaling maintains vascular tone; its pharmacological inhibition leads to increased cGMP levels and prolonged vasodilation, making it the primary target for treating erectile dysfunction and pulmonary arterial hypertension. The anti-PDE5A antibody (PD134) has been tested in Immunohistochemistry (IHC), Immunoprecipitation (IP) and Western Blot (WB).
Gene Expression
Subcellular Location
Figure 1 IF staining of human cell line SiHa
Immunofluorescent staining of human cell line SiHa shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4729/1921_F8_7_cr5cb46231d0b24_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Neuronal cells Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12873/30148_B_8_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12873/30148_A_7_3.jpg
Normal Tissue
Figure 4 Liver
Cholangiocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12873/30148_A_7_4.jpg
Normal Tissue
Figure 5 Pancreas
Exocrine glandular cells Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous Pancreatic endocrine cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12873/30148_A_1_3.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Leydig cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/12873/30148_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (BJ hTERT+, HHSteC, hTERT-RPE1)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000138735-PDE5A
❮
❯
Specifications
- Host Species
- Mouse
- Type
- Mouse IgG2a
- Specificity
- Human PDE5A
- Species Reactivity
- Human
- Clone
- PD134
- Applications
- IHC, IP, WB
- Conjugate
- The unconjugated, Biotin and FITC formats are available.
Product Property
- Clonality
- Monoclonal
- Purification
- Affinity purified
- Format
- Liquid
- Buffer
- PBS
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB (1/500-1/1000), IHC (1/100-1/200), IP (1/100-1/200)
Target
- Alternative Names
- Phosphodiesterase 5A; Phosphodiesterase 5A, CGMP-Specific; EC 3.1.4.35; CGB-PDE; PDE5; CGMP-Binding CGMP-Specific 3,5-Cyclic Nucleotide Phosphodiesterase; CGMP-Binding CGMP-Specific Phosphodiesterase;
- Gene ID
- 8654
- UniProt ID
- O76074
- Long Name
- Phosphodiesterase 5A
- Post Translation Modifications
- Unmodified
REVIEWS AND Q&AS
CITATIONS
RESOURCES
DOWNLOADS
RELATED PRODUCTS
Inquiry
Navs
Customer Review
There are currently no Customer reviews or questions for VS-0326-YC134. Click the button above to contact us or submit your feedback about this product.
Submit Your Publication
Published with our product? Submit your paper and receive a 10% discount on your next order! Share your research to earn exclusive rewards.
Related Signaling Pathways
cAMP Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "PDE5A"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1045MZ | Recombinant Mouse Anti-Human PDE5A Antibody | WB | Mouse antibody |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1597 | Anti-PDE5A Immunohistochemistry Kit | IHC |
Specific Inquiry
Add to compare
See Our Custom Production in Action
Popular Products
Application: IF, IP, Neut, FuncS, ELISA, FC, ICC
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: Neut, ELISA, IF, IP, FuncS, FC, WB
Application: WB, ELISA, FC, IP, FuncS, IF, Neut
Application: ELISA, FC, IP, FuncS, IF, Neut, ICC
Application: ELISA, FC, IP, FuncS, IF, Neut, WB
Application: ELISA, IP, FC, FuncS, Neut, IF, ICC
Application: Neut, ELISA, FuncS
Application: WB, Neut, FuncS
For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
Send Inquiry
This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.
