Anti-PDK1 Antibody Prodrug, Protease Activated (VS-1025-YC107)
CAT#: VS-1025-YC107
The Anti-PDK1 Antibody Prodrug, Protease Activated (VS-1025-YC107) is an antibody prodrug comprised of a recombinant human antibody targeting human PDK1, linked to a masking peptide that conceals the active antigen-binding site via a protease-cleavable linker. The mask peptide is recombinantly attached to the amino terminus of either the light or heavy chain of the antibody, designed to block access to the antigen-binding site and physically prevent the antibody from binding to PDK1 protein. Once the masking peptide is cleaved by matrix metalloproteinases (MMPs), the prodrug is activated, allowing the unmasked monoclonal antibody component to bind to PDK1 effectively. PDK1 (Phosphoinositide-Dependent Kinase 1) is a critical enzyme that acts as a central node in the highly oncogenic PI3K/AKT signaling pathway. PDK1 directly activates the pro-survival kinase AKT, making it essential for the anti-apoptotic and pro-proliferative signaling that is constitutively active in numerous cancers.
Gene Expression
Normal Tissue
Figure 1 IHC staining of human duodenum
Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_7_2_selected.jpg
Normal Tissue
Figure 2 Cerebral cortex
Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_B_7_5.jpg
Normal Tissue
Figure 3 Colon
Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_8_3.jpg
Normal Tissue
Figure 4 Liver
Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_7_4.jpg
Normal Tissue
Figure 5 Kidney
Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_9_5.jpg
Normal Tissue
Figure 6 Testis
Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_5_6.jpg
RNA Expression
Figure 7 RNA cell line category: Cell line enhanced (U-266/70, U-87 MG)
Cell lines ordered by descending RNA expression order
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152256-PDK1
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Specifications
- Host Animal
- Human
- Applications
- In situ zymography (ISZ), Cytotoxicity assay (Cyt), Functional assay
- Specificity
- Human PDK1
- Target
- PDK1
- Species Reactivity
- Human
- Isotype
- IgG1
- Masking Approach
- Affinity mask
- Mask
- A synthetic peptide
- Protease Cleavable Linker
- MMP-cleavable linker
- Protease Substrate
- MMPs
- Purification
- Protein A purified
- Buffer
- PBS, pH 7.4
- Preservative
- No preservatives
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Target
- Introduction
- Pyruvate dehydrogenase (PDH) is a mitochondrial multienzyme complex that catalyzes the oxidative decarboxylation of pyruvate and is one of the major enzymes responsible for the regulation of homeostasis of carbohydrate fuels in mammals. The enzymatic activity is regulated by a phosphorylation/dephosphorylation cycle. Phosphorylation of PDH by a specific pyruvate dehydrogenase kinase (PDK) results in inactivation. Multiple alternatively spliced transcript variants have been found for this gene.
- Alternative Names
- Pyruvate dehydrogenase kinase 1
- Gene ID
- 5163
- UniProt ID
- O15530
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Related Diseases
Endometrial Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Insulin Resistance
Related Signaling Pathways
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PDK1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2840z | Mouse Anti-PDK1 Recombinant Antibody (clone 34H4) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| MOB-1926MZ | Recombinant Mouse Anti-Human PDK1 Antibody (clone 5B22) | ELISA, FC, ICC, IF, IHC-P, WB | Mouse antibody |
| HPAB-0015-CN | Human Anti-PDK1 Recombinant Antibody (clone 2B9) | IF, ELISA, FC | Human IgG1 |
| MRO-1198-CN | Recombinant Rabbit Anti-PDK1 Monoclonal Antibody (JA67-30) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-WK265 | Rabbit Anti-PDK1 Recombinant Antibody (clone JA67-30) | WB, ELISA | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2644 | Hi-Affi™ Recombinant Rabbit Anti-PDK1 Monoclonal Antibody (DS2644AB) | WB, IP | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0015-CN-S(P) | Human Anti-PDK1 Recombinant Antibody (clone 2B9); scFv Fragment | IF, ELISA, FC | Human scFv |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| HPAB-0015-CN-F(E) | Human Anti-PDK1 Recombinant Antibody (clone 2B9); Fab Fragment | IF, ELISA, FC | Human Fab |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY374 | Mouse Anti-NHP PDK1 Recombinant Antibody (clone 4A11) | WB, IHC, ELISA | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC889 | CytoStream™ Rabbit Anti-PDK1 Recombinant Antibody (VS13-YC889) | WB, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1606 | Anti-PDK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5290 | Anti-Mouse PDK1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY5289 | Anti-Human PDK1 Immunohistochemistry Kit | IHC |
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