Mouse Anti-NHP PDK1 Recombinant Antibody (clone 4A11) (CAT#: VS-1024-XY374)
This mouse-derived antibody specifically targets PDK1, utilizing a purified recombinant fragment of human PDK1 as its immunogen, which ensures optimal reactivity with human, mouse, non-human primate, and rat samples. Its versatile applications in Western Blotting (WB), Immunohistochemistry (IHC), and ELISA provide essential insights into the regulatory mechanisms of PDK1 in various biological contexts. Known for its high specificity and reliability, this antibody is a vital tool for researchers aiming to elucidate the pathways of cellular signaling and metabolism, advancing the understanding of numerous physiological processes and diseases.
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_7_2_selected.jpg
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_7_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_5_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_5_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (U-266/70, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152256-PDK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152256-PDK1
Specifications
- Immunogen
- Purified recombinant fragment of human PDK1
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- PDK1
- Species Reactivity
- Human, Mouse, Non-human primate, Rat
- Clone
- 4A11
- Applications
- WB, IHC, ELISA
Product Property
- Purification
- Protein A purified
- Purity
- >95%, determined by SDS-PAGE
- Format
- Liquid
- Buffer
- PBS, pH 7.4
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- WB: 1:500-1:1000, IHC: 1:200-1:800, ELISA: 1:8000
Target
- Alternative Names
- Pyruvate Dehydrogenase Kinase 1; Pyruvate Dehydrogenase Kinase; Isoenzyme 1; Pyruvate Dehydrogenase Kinase; Isozyme 1; Pyruvate Dehydrogenase Kinase Isoform 1; PDH Kinase 1; EC 2.7.11; PDHK1;
- Gene ID
- 5163
- UniProt ID
- Q15118
Recommended Products
Recommended Dilution Buffer
Isotype Control
Secondary Antibody
Related Resources
Endometrial Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Insulin Resistance
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Fab Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2840z | Mouse Anti-PDK1 Recombinant Antibody (clone 34H4) | WB, ELISA, FC, ICC, IF, IHC | Mouse IgG1 |
| HPAB-0015-CN | Human Anti-PDK1 Recombinant Antibody (clone 2B9) | IF, ELISA, FC | Human IgG1 |
| MRO-1198-CN | Recombinant Rabbit Anti-PDK1 Monoclonal Antibody (JA67-30) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-WK265 | Rabbit Anti-PDK1 Recombinant Antibody (clone JA67-30) | WB, ELISA | Rabbit IgG |
| VS3-XY1232 | Mouse Anti-PDK1 Recombinant Antibody (clone 4A11) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
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For research use only. Not intended for any clinical use. No products from Creative Biolabs may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative Biolabs.
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