CytoStream™ Rabbit Anti-PDK1 Recombinant Antibody (VS13-YC889) (CAT#: VS13-YC889)
The CytoStream™ Rabbit Anti-PDK1 Recombinant Antibody is specific for human, mouse and rat PDK1. PDK1 encodes a kinase regulating PDH activity via phosphorylation, a key enzyme governing carbohydrate fuel homeostasis, showing multiple spliced forms. The anti-PDK1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specific Inquiry
Normal Tissue
RNA Expression
Figure 1 IHC staining of human duodenum
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_7_2_selected.jpg
(Immunohistochemical staining of human duodenum shows moderate cytoplasmic positivity in glandular cells.)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_7_2_selected.jpg
Figure 2 Cerebral cortex
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_B_7_5.jpg
(Endothelial cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous Neuropil Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_B_7_5.jpg
Figure 3 Colon
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_8_3.jpg
(Endothelial cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Glandular cells Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous Peripheral nerve/ganglion Staining: High Intensity: Strong Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_8_3.jpg
Figure 4 Liver
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_7_4.jpg
(Hepatocytes Staining: Medium Intensity: Moderate Quantity:>75% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_7_4.jpg
Figure 5 Kidney
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_9_5.jpg
(Cells in tubules Staining: Medium Intensity: Moderate Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/17554/40219_A_9_5.jpg
Figure 6 Testis
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_5_6.jpg
(Cells in seminiferous ducts Staining: Low Intensity: Moderate Quantity: <25% Location: Cytoplasmic/ membranous Leydig cells Staining: Low Intensity: Weak Quantity: 75%-25% Location: Cytoplasmic/ membranous)
* Image credit: Human Protein Atlas v21.proteinatlas.org/images/27376/57115_A_5_6.jpg
Figure 7 RNA cell line category: Cell line enhanced (U-266/70, U-87 MG)
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152256-PDK1
(Cell lines ordered by descending RNA expression order)
* Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000152256-PDK1
Specifications
- Immunogen
- Recombinant human PDK1, aa 16-65.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PDK1
- Species Reactivity
- Human, Mouse, Rat
- Applications
- WB, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/500-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Pyruvate Dehydrogenase Kinase 1; EC 2.7.11.2; Pyruvate Dehydrogenase Kinase, Isoenzyme 1; Pyruvate Dehydrogenase Kinase, Isozyme 1; Pyruvate Dehydrogenase Kinase Isoform 1; PDH Kinase 1; EC 2.7.11; PDHK1;
- Gene ID
- 5163
- UniProt ID
- Q15118
- Long Name
- Pyruvate Dehydrogenase Kinase 1
- Cellular Localization
- Mitochondrion matrix
- Function
- Signal transduction
Recommended Products
Secondary Antibody
Isotype Control
Related Resources
Endometrial Cancer
Non-small Cell Lung Cancer
Prostate Cancer
Insulin Resistance
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
PPAR Signaling Pathway
Ras Signaling Pathway
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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Non-Human Primate (NHP) Antibody Products
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1926MZ | Recombinant Mouse Anti-Human PDK1 Antibody (clone 5B22) | ELISA, FC, ICC/IF, IHC-P, WB | Mouse antibody |
| MRO-1198-CN | Recombinant Rabbit Anti-PDK1 Monoclonal Antibody (JA67-30) | WB, IHC, IP, FC | Rabbit IgG |
| VS3-WK265 | Rabbit Anti-PDK1 Recombinant Antibody (clone JA67-30) | WB, ELISA | Rabbit IgG |
| VS3-XY1232 | Mouse Anti-PDK1 Recombinant Antibody (clone 4A11) | ELISA, WB, IHC, FC, ICC | Mouse IgG1 |
| VS3-WK1313 | Rabbit Anti-PDK1 Recombinant Antibody (VS3-WK1313) | ELISA, IHC, WB | Rabbit IgG |
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