Rabbit Anti-Phospho-PTEN (S380) Recombinant Antibody (clone 4D10)
CAT#: ZG-0527U
Rabbit Anti-Phospho-PTEN (S380) Antibody (clone 4D10) displayed high specificity against the PTEN protein of Human.
Tested Data
Gene Expression
WB
Figure 1 Rabbit Anti-Phospho-PTEN (S380) Antibody (ZG-0527U) in WB
Western Blot
Positive WB detected in HepG2 whole cell lysate
All lanes Phospho-PTEN antibody at 1.9μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 54 KDa
Observed band size: 54 KDa
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line MCF7
Immunofluorescent staining of human cell line MCF7 shows localization to nucleoplasm.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human testis
Immunohistochemical staining of human testis shows cytoplasmic positivity in cells in seminiferous ducts.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/31335/134179_A_5_6_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Neuropil
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_B_8_5.jpg
Normal Tissue
Figure 4 Lymph node
Germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous nuclear
Non-germinal center cells
Staining: Low
Intensity: Weak
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4076/12125_A_8_8.jpg
RNA Expression
Figure 5 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000171862-PTEN/subcellular
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-PTEN (S380)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-PTEN (S380)
- Species Reactivity
- Human
- Clone
- 4D10
- Applications
- ELISA, WB
- Conjugate
- Unconjugated
- Related Disease
- Cowden Syndrome 1 and Macrocephaly/Autism Syndrome.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot. Specific conditions should be optimized by the end user.
WB:1:500-1:5000
Target
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Related Diseases
Endometrial Cancer
Related Signaling Pathways
mTOR Signaling Pathway
PI3K-Akt Signaling Pathway
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
See other products for "PTEN"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-1640z | Mouse Anti-PTEN Recombinant Antibody (clone 27C11) | WB, ELISA, FC, ICC, IF | Mouse IgG1 |
| VS3-QX925 | Mouse Anti-PTEN Recombinant Antibody (clone 4G4-4H9-1E10) | IHC | Mouse IgG1 |
| VS3-QX926 | Mouse Anti-PTEN Recombinant Antibody (clone 7H2-6G9-6H5) | IHC | Mouse IgG1 |
| VS3-QX927 | Mouse Anti-PTEN Recombinant Antibody (clone 1B8) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS13-YC954 | Rabbit Anti-PTEN Recombinant Antibody (VS13-YC954) | WB, ICC, IF, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| IAB-B021(A) | Recombinant Anti-mouse PTEN Intrabody [(D-Arg)9] | PCA, WB, FuncS | scFv-(D-Arg)9 |
| IAB-B021(G) | Recombinant Anti-mouse PTEN Intrabody [+36 GFP] | FACS, ELISA, IF, FuncS | scFv-(+36GFP) |
| IAB-B021(T) | Recombinant Anti-mouse PTEN Intrabody [Tat] | FACS, ICC, FuncS | scFv-Tat |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-2915 | Hi-Affi™ Recombinant Rabbit Anti-PTEN Monoclonal Antibody (DS2915AB) | ICC, IHC-P, IP, WB | IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY183 | CytoStream™ Mouse Anti-PTEN Recombinant Antibody (VS-0225-XY183) | FC | Mouse IgG1, kappa |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY1816 | Anti-PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5823 | Anti-Mouse PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5822 | Anti-Human PTEN Immunohistochemistry Kit | IHC | |
| VS-0525-XY5824 | Anti-Rat PTEN Immunohistochemistry Kit | IHC |
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