Rabbit Anti-Phospho-YAP1 (S127) Recombinant Antibody (clone 3F3) (CAT#: ZG-0623U)
Rabbit Anti-Phospho-YAP1 (S127) Antibody (clone 3F3) displayed high specificity against the YAP1 protein of Human.
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Custom Production
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WB
Figure 1 Rabbit Anti-Phospho-YAP1 (S127) Antibody (ZG-0623U) in WB
Western Blot
Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-YAP1 antibody at 0.83μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 KDa
Observed band size: 65 KDa
IHC
Figure 2 Rabbit Anti-Phospho-YAP1 (S127) Antibody (ZG-0623U) in IHC
IHC image of ZG-0623U diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-YAP1 (S127)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-YAP1 (S127)
- Species Reactivity
- Human
- Clone
- 3F3
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/Palate, And/Or Mental Retardation and Coloboma Of Macula.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
- Alternative Names
- Transcriptional coactivator YAP1; Protein yorkie homolog; Yes-associated protein YAP65 homolog; YAP1; YAP65
- Gene ID
- 10413
- UniProt ID
- P46937
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0305MC | Rcombinant Anti-Human YAP1 Antibody. | WB, ELISA, ICC/IF, IHC, IHC-P, S-ELISA | |
| MOB-0306MC | Rabbit Anti-Phospho-YAP (Ser127) Antibody | WB |
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For Research Use Only. Not For Clinical Use.
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