Rabbit Anti-Phospho-YAP1 (S127) Recombinant Antibody (clone 3F3)
CAT#: ZG-0623U
Rabbit Anti-Phospho-YAP1 (S127) Antibody (clone 3F3) displayed high specificity against the YAP1 protein of Human.
Tested Data
WB
Figure 1 Rabbit Anti-Phospho-YAP1 (S127) Antibody (ZG-0623U) in WB
Western Blot
Positive WB detected in HepG2 whole cell lysate(treated with Calyculin A or not)
All lanes Phospho-YAP1 antibody at 0.83μg/ml
Secondary
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 65 KDa
Observed band size: 65 KDa
IHC
Figure 2 Rabbit Anti-Phospho-YAP1 (S127) Antibody (ZG-0623U) in IHC
IHC image of ZG-0623U diluted at 1:100 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Specifications
- Immunogen
- A synthesized peptide derived from human Phospho-YAP1 (S127)
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human Phospho-YAP1 (S127)
- Species Reactivity
- Human
- Clone
- 3F3
- Applications
- ELISA, WB, IHC
- Conjugate
- Unconjugated
- Related Disease
- Coloboma, Ocular, With Or Without Hearing Impairment, Cleft Lip/Palate, And/Or Mental Retardation and Coloboma Of Macula.
Product Property
- Purification
- Affinity chromatography purified
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry. Specific conditions should be optimized by the end user.
WB:1:500-1:5000, IHC:1:50-1:200
Target
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
See other products for "YAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0305MC | Rcombinant Anti-Human YAP1 Antibody. | WB, ELISA, ICC, IF, IHC, IHC-P, S-ELISA | |
| MOB-0306MC | Rabbit Anti-Phospho-YAP (Ser127) Antibody | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3899 | Rabbit Anti-YAP1 Recombinant Antibody (clone DS3899AB) | IHC-P, WB | Rabbit IgG |
| MOR-0048-FY | Rabbit Anti-YAP1 Recombinant Antibody (clone AFY0019) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2393-CN | Rabbit Anti-YAP1 Recombinant Antibody (clone CBACN-678) | WB, IHC | Rabbit IgG |
| VS3-QX1256 | Mouse Anti-YAP1 Recombinant Antibody (clone 1A12) | WB, IHC, ICC, FC, ELISA | Mouse IgG1 |
| VS3-CJ978 | Rabbit Anti-YAP1 Recombinant Antibody (VS3-CJ978) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ979 | Rabbit Anti-YAP1 (phospho S127) Recombinant Antibody (VS3-CJ979) | WB, IHC | Rabbit IgG |
| VS-0723-WK77 | Human Anti-YAP1 Recombinant Antibody (VS-0723-WK77) | FC | Chimeric (rabbit/human) IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY277 | AbPlus™ Anti-YAP1 Magnetic Beads (CBACN-585) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS13-YC1232 | CytoStream™ Rabbit Anti-YAP1 Recombinant Antibody (VS13-YC1232) | WB, ICC, IF, IHC-P, IP, FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2503 | Anti-YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7974 | Anti-Mouse YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7973 | Anti-Human YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7975 | Anti-Rat YAP1 Immunohistochemistry Kit | IHC |
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