CytoStream™ Rabbit Anti-YAP1 Recombinant Antibody (VS13-YC1232) (CAT#: VS13-YC1232)

• Immunogen
• Recombinant human YAP1, aa 421-470.

• Host Species
• Rabbit

• Type
• Rabbit IgG

• Specificity
• Human YAP1

• Species Reactivity
• Human

• Applications
• WB, ICC, IF, IHC-P, IP, FC

• Conjugate
• NONE

• Clonality
• Monoclonal

• Purification
• Protein A purified

• Format
• Liquid

• Concentration
• Please refer to the vial label for the specific concentration.

• Buffer
• TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.

• Preservative
• 0.05% Sodium azide

• Storage
• Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

• Shipping
• Shipping with ice pack.

• Application Notes
• Western blot (WB): 1/1000-1/2000
  Immunocytochemistry (ICC): 1/50-1/200
  Immunofluorescence (IF): 1/50-1/200
  Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
  Flow cytometry (FC): 1/50-1/100

• Alternative Names
• Yes Associated Protein 1; Yes-Associated Protein YAP65 Homolog; Yes-Associated Protein 1; Protein Yorkie Homolog; YAP65; Transcriptional Coactivator YAP1; Yes-Associated Protein 1, 65kDa; 5 KDa Yes-Associated Protein;

• Gene ID
• 10413

• UniProt ID
• P46937

• Long Name
• Yes Associated Protein 1

• Sequence Similarities
• Belongs to the YAP1 family.

• Cellular Localization
• Nucleus; Cytoplasm

• Protein Refseq
• NP_001123617.1; NP_001181973.1; NP_001181974.1

• Function
• Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097).
  The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288).
  Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702).
  Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288).
  The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
  Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
  In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
  Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
  Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).

Secondary Antibody

• 2ND-059CQ
• Goat Anti-Rabbit IgG (H&L) Antibody

• 2ND-061CQ
• Sheep Anti-Rabbit IgG (H&L) Antibody

Isotype Control

• MOB-208CQ
• Rabbit IgG Isotype Control Antibody

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