CytoStream™ Rabbit Anti-YAP1 Recombinant Antibody (VS13-YC1232)
CAT#: VS13-YC1232
The CytoStream™ Rabbit Anti-YAP1 Recombinant Antibody is specific for human YAP1. YAP1, a key effector of the Hippo signaling pathway, regulates development and cancer progression and serves as a potential cancer therapy target. The anti-YAP1 antibody was developed and selected by our CytoStream™ platform and the mAb is recommended for use in flow cytometry staining (1/50-1/100).
Specifications
- Immunogen
- Recombinant human YAP1, aa 421-470.
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human YAP1
- Species Reactivity
- Human
- Applications
- WB, ICC, IF, IHC-P, IP, FC
- Conjugate
- NONE
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- TBS (pH 7.4) containing 40% Glycerol and 0.05% BSA.
- Preservative
- 0.05% Sodium azide
- Storage
- Store at 4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
- Shipping
- Shipping with ice pack.
Applications
- Application Notes
- Western blot (WB): 1/1000-1/2000
Immunocytochemistry (ICC): 1/50-1/200
Immunofluorescence (IF): 1/50-1/200
Immunohistochemistry-paraffin (IHC-P): 1/50-1/200
Flow cytometry (FC): 1/50-1/100
Target
- Alternative Names
- Yes Associated Protein 1; Yes-Associated Protein YAP65 Homolog; Yes-Associated Protein 1; Protein Yorkie Homolog; YAP65; Transcriptional Coactivator YAP1; Yes-Associated Protein 1, 65kDa; 5 KDa Yes-Associated Protein;
- Gene ID
- 10413
- UniProt ID
- P46937
- Long Name
- Yes Associated Protein 1
- Sequence Similarities
- Belongs to the YAP1 family.
- Cellular Localization
- Nucleus; Cytoplasm
- Protein Refseq
- NP_001123617.1; NP_001181973.1; NP_001181974.1
- Function
- Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis (PubMed:17974916, PubMed:18280240, PubMed:18579750, PubMed:21364637, PubMed:30447097).
The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ (PubMed:18158288).
Plays a key role in tissue tension and 3D tissue shape by regulating cortical actomyosin network formation. Acts via ARHGAP18, a Rho GTPase activating protein that suppresses F-actin polymerization (PubMed:25778702).
Plays a key role in controlling cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration (PubMed:18158288).
The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction (PubMed:18579750).
Suppresses ciliogenesis via acting as a transcriptional corepressor of the TEAD4 target genes AURKA and PLK1 (PubMed:25849865).
In conjunction with WWTR1, involved in the regulation of TGFB1-dependent SMAD2 and SMAD3 nuclear accumulation (By similarity).
Isoform 2: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
Isoform 3: Activates the C-terminal fragment (CTF) of ERBB4 (isoform 3).
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
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Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Secondary Antibody
- CAT
- Product Name
Isotype Control
- CAT
- Product Name
See other products for "YAP1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-0305MC | Rcombinant Anti-Human YAP1 Antibody. | WB, ELISA, ICC, IF, IHC, IHC-P, S-ELISA | |
| MOB-0306MC | Rabbit Anti-Phospho-YAP (Ser127) Antibody | WB |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOR-3899 | Rabbit Anti-YAP1 Recombinant Antibody (clone DS3899AB) | IHC-P, WB | Rabbit IgG |
| MOR-0048-FY | Rabbit Anti-YAP1 Recombinant Antibody (clone AFY0019) | ICC, IHC-P, WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS3-CJ978 | Rabbit Anti-YAP1 Recombinant Antibody (VS3-CJ978) | WB, ICC, IF, IHC, IP, FC | Rabbit IgG |
| VS3-CJ979 | Rabbit Anti-YAP1 (phospho S127) Recombinant Antibody (VS3-CJ979) | WB, IHC | Rabbit IgG |
| VS3-FY1565 | Recombinant Rabbit Anti-YAP1 Antibody (clone R06-6H7) | WB, IHC-P, IP | Rabbit IgG |
| VS3-FY1566 | Recombinant Rabbit Anti-YAP1 Antibody (clone R06-5E9) | WB, ICC, IF | Rabbit IgG |
| VS3-FY1567 | Recombinant Rabbit Anti-YAP1 (phospho Ser127) Antibody (clone R07-6C2) | WB, IHC-P | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0424-XY277 | AbPlus™ Anti-YAP1 Magnetic Beads (CBACN-585) | IP, Protein Purification |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0325-XY2503 | Anti-YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7974 | Anti-Mouse YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7973 | Anti-Human YAP1 Immunohistochemistry Kit | IHC | |
| VS-0525-XY7975 | Anti-Rat YAP1 Immunohistochemistry Kit | IHC |
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