AibGenesis™ Rabbit Anti-PKM Recombinant Antibody (AibG-Rb-PK1704)
CAT#: VS-0326-XY529
Highly specific, this recombinant rabbit antibody binds PKM, a pyruvate kinase isoform critical for glycolysis and energy metabolism. It is cross-species reactive and suitable for studies in metabolic regulation. Altered activity may affect ATP production.
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/283_B10_1_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to cytosol.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/if_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IHC staining of human fallopian tube
Immunohistochemical staining of human fallopian tube shows moderate cytoplasmic and membranous positivity in glandular cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/ihc_selected.jpg
Subcellular Location and Protein Expression
Figure 4 IHC staining of human urinary bladder
Immunohistochemical staining of human urinary bladder shows strong cytoplasmic positivity in urothelial cells.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/19421/ihc_selected.jpg
Normal Tissue
Figure 5 Cerebral cortex
Neuronal cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous Neuropil
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_B_8_5.jpg
Normal Tissue
Figure 6 Colon
Endothelial cells
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
Glandular cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Peripheral nerve/ganglion
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_3.jpg
Normal Tissue
Figure 7 Liver
Cholangiocytes
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_4.jpg
Normal Tissue
Figure 8 Kidney
Cells in tubules
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_9_5.jpg
Normal Tissue
Figure 9 Testis
Cells in seminiferous ducts
Staining: Medium
Intensity: Moderate
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_4_6.jpg
Normal Tissue
Figure 10 Lymph node
Germinal center cells
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/29501/60955_A_8_8.jpg
RNA Expression
Figure 11 RNA cell line category: Low cell line specificity
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000067225-PKM
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- PKM
- Species Reactivity
- Human, Mouse, Rat
- Clone
- AibG-Rb-PK1704
- Applications
- Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Enzyme-linked Immunosorbent Assay, Immunoprecipitation
- Conjugate
- Unconjugated
Product Property
- Clonality
- Monoclonal
- Purification
- Protein A purified
- Format
- Liquid
- Buffer
- PBS, 50% glycerol and 0.05% protective protein.
- Preservative
- 0.05% Proclin 300
- Storage
- Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1:1000-1:5000, Immunohistochemistry: 1:200-1:1000, Immunocytochemistry/Immunofluorescence: 1:200-1:1000, ELISA: 1:5000-1:20000, Immunoprecipitation: 1:50-1:200
Target
- Alternative Names
- PKM, OIP3, PK2, PK3, PKM2, Pyruvate kinase isozymes M1/M2, Cytosolic thyroid hormone-binding protein, CTHBP, Opa-interacting protein 3, OIP-3, Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, THBP1, Tu
- Gene ID
- 5315
- UniProt ID
- P14618
- Long Name
- Pyruvate kinase
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PKM"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2556z | Mouse Anti-PKM Recombinant Antibody (clone 34C9) | WB, ICC, IF | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MRO-2125-CN | Rabbit Anti-PKM Polyclonal Antibody (MRO-2125-CN) | WB, IF, IHC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0378C | Mouse Anti-PKM Recombinant Antibody (ZG-0378C) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0379C | Mouse Anti-PKM Recombinant Antibody (ZG-0379C) | WB, ICC | Mouse IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0247U | Mouse Anti-PKM Recombinant Antibody (clone 6C3C7) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG1 |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-1024-XY393 | Rabbit Anti-NHP PKM Recombinant Antibody (VS-1024-XY393) | WB, IF | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0225-XY178 | CytoStream™ Rabbit Anti-PKM Recombinant Antibody (VS-0225-XY178) | FC | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5443 | Anti-PKM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5444 | Anti-Rat PKM Immunohistochemistry Kit | IHC |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5445 | Anti-Mouse PKM Immunohistochemistry Kit | IHC |
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