Mouse Anti-PKM Recombinant Antibody (clone 4C8E12) (CAT#: ZG-0248U)
Mouse anti-PKM antibody (clone 4C8E12) displayed high specificity against the PKM protein of Human and Mouse.
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WB
Figure 1 Mouse Anti-PKM Antibody (ZG-0248U) in WB
Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PKM antibody at 1: 5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 58 kDa
Observed band size: 58 KDa
Exposure time: 1min
IHC
Figure 2 Mouse Anti-PKM Antibody (ZG-0248U) in IHC
IHC image of ZG-0248U diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
IF
Figure 3 Mouse Anti-PKM Antibody (ZG-0248U) in IF
Immunofluorescence staining of HepG2 cells with ZG-0248U at 1:215, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Mouse IgG(H+L).
FC
Figure 4 Mouse Anti-PKM Antibody (ZG-0248U) in FC
Overlay histogram showing Hela cells stained with ZG-0248U (red line) at 1:50. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4°C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4°C. Isotype control antibody (green line) was used under the same conditions. Acquisition of>10,000 events was performed.
Specifications
- Immunogen
- Recombinant Human Pyruvate kinase PKM protein (2-531AA)
- Host Species
- Mouse
- Type
- Mouse IgG1
- Specificity
- Human PKM
- Species Reactivity
- Human, Mouse
- Clone
- 4C8E12
- Applications
- ELISA, WB, IHC, IF, FC, IP
- Conjugate
- Unconjugated
- Related Disease
- Pyruvate Kinase Deficiency Of Red Cells and Anterior Dislocation Of Lens
Product Property
- Purification
- Protein G purified
- Purity
- >95% as determined by SDS-PAGE
- Buffer
- PBS, pH 7.4, 0.03% Proclin 300, 50% glycerol.
- Preservative
- 0.03% Proclin 300
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation. Specific conditions should be optimized by the end user.
WB:1:4000-1:512000, IHC: 1:500-1:1000, IF: 1:150-1:300, FC: 1:50-1:100, IP: 1µl-4µl
Target
- Alternative Names
- PK3; TCB; p58; OIP3; PKM2; CTHBP; THBP1; HEL-S-30; pyruvate kinase PKM; OPA-interacting protein 3; PK, muscle type; cytosolic thyroid hormone-binding protein; epididymis secretory protein Li 30; pyruvate kinase 2/3; pyruvate kinase isozymes M1/M2; pyruvate kinase muscle isozyme; pyruvate kinase, muscle; thyroid hormone-binding protein 1; thyroid hormone-binding protein, cytosolic; tumor M2-PK
- Gene ID
- 5315
- UniProt ID
- P14618
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Downloads
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| CAT | Product Name | Application | Type |
|---|---|---|---|
| ZG-0247U | Mouse Anti-PKM Recombinant Antibody (clone 6C3C7) | ELISA, WB, IHC, IF, FC, IP | Mouse IgG1 |
| ZG-0513U | Rabbit Anti-PKM Recombinant Antibody (clone 7B2) | ELISA, WB, IHC, IF, FC, IP | Rabbit IgG |
| VS3-QX879 | Mouse Anti-PKM Recombinant Antibody (clone 1F2-F4-H11) | WB, IF, IP | Mouse IgG2b |
| VS3-QX880 | Mouse Anti-PKM Recombinant Antibody (clone 1A7-G6-H6) | WB, IF | Mouse IgG1 |
| VS3-XY3466 | Rabbit Anti-PKM Recombinant Antibody (clone R09-7E7) | WB, IHC-P, ICC, IF, FC | Rabbit IgG |
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