Mouse Anti-PLAU Recombinant Antibody (clone 6A7C6)

Name: Mouse Anti-PLAU Recombinant Antibody (clone 6A7C6)
SKU: VS7-0425-WR624
Rating: 5 (1 reviews)

CAT#: VS7-0425-WR624

This product is a mouse IgG1 antibody that binds to human PLAU. The antibody 6A7C6 recognizes specific amino acid domain of human PLAU (AA: 107-379). It can be used for FC assay.

Gene Expression

Subcellular Location and Protein Expression Figure 1 IF staining of human cell line RPTEC TERT1

Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1789_A4_1_cr5bacc2acbd568_selected.jpg

Subcellular Location and Protein Expression Figure 2 IHC staining of human placenta

Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_2_7_selected.jpg

Subcellular Location and Protein Expression Figure 3 IF staining of human cell line U-2 OS

Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1886_D2_1_blue_red_green.jpg

Subcellular Location and Protein Expression Figure 4 IF staining of human cell line U-251 MG

Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1819_G1_4_cr5bacc1ca11170_blue_red_green.jpg

Normal Tissue Figure 5 Cerebral cortex

Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_B_9_5.jpg

Normal Tissue Figure 6 Colon

Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:Low
Intensity: Moderate
Quantity: <25%
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_8_3.jpg

Normal Tissue Figure 7 Kidney

Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Collecting ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Distal tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_9_5.jpg

Normal Tissue Figure 8 Testis

Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_5_6.jpg

Normal Tissue Figure 9 Prostate

Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_2_5.jpg

RNA Expression Figure 10 RNA cell line category: Cell line enhanced (CAPAN-2, LHCN-M2, RPTEC TERT1, U-251 MG)

Cell lines ordered by descending RNA expression order.

* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122861-PLAU

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Specifications

Immunogen
Recombinant human PLAU (AA: 107-379) fragment expressed in E. Coli.

Host Species
Mouse

Type
Mouse IgG1

Specificity
Human PLAU

Species Reactivity
Human

Clone
6A7C6

Molecule Class
Serine Protease

Applications
FC

Conjugate
Unconjugated

Related Disease
Quebec platelet disorder (QPD)

Product Property

Clonality
Monoclonal

Purity
>95% as determined by SDS-PAGE and SEC-HPLC analysis

Format
Liquid

Concentration
Please refer to the vial label for the specific concentration.

Buffer
PBS

Preservative
0.05% sodium azide

Storage
Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.

Shipping
Ice packs

Applications

Application Notes
Western Blot: 1:500 - 1:2000
Flow Cytometry: 1:200 - 1:400
Enzyme-linked Immunosorbent Assay: 1:10000

Target

Alternative Names
PLAU; plasminogen activator, urokinase; ATF; QPD; UPA; URK; u-PA; BDPLT5; urokinase-type plasminogen activator; U-plasminogen activator; plasminogen activator, urinary

Gene ID
5328

UniProt ID
P00749

Long Name
Plasminogen Activator, Urokinase

Cellular Localization
Secreted

Post Translation Modifications
Disulfide bond; Glycoprotein; Phosphoprotein; Zymogen

Protein Refseq
NP_001138503.2

Function
Hydrolase; Protease; Serine protease

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Product Notes

This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:

• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production

See more details about Hi-Affi™ recombinant antibody benefits.

Datasheet

Datasheet - VS7-0425-WR624.pdf

MSDS

MSDS - VS7-0425-WR624.pdf

COA

Certificate of Analysis Lookup

To download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.

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