Rabbit Anti-PLAU Recombinant Antibody (clone 10E5)
CAT#: ZG-0079U
The purified Rabbit Anti-PLAU Recombinant Antibody (clone 10E5) displayed high specificity against the PLAU protein of human.
Tested Data
Gene Expression
IHC
Figure 1 Rabbit Anti-PLAU Antibody (ZG-0079U) in IHC
IHC image of ZG-0079U diluted at 1:100 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB.
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Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line RPTEC TERT1
Immunofluorescent staining of human cell line RPTEC TERT1 shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1789_A4_1_cr5bacc2acbd568_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human placenta
Immunohistochemical staining of human placenta shows strong cytoplasmic and membranous positivity in trophoblastic cells.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_2_7_selected.jpg
Subcellular Location and Protein Expression
Figure 3 IF staining of human cell line U-2 OS
Immunofluorescent staining of human cell line U-2 OS shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1886_D2_1_blue_red_green.jpg
Subcellular Location and Protein Expression
Figure 4 IF staining of human cell line U-251 MG
Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/1819_G1_4_cr5bacc1ca11170_blue_red_green.jpg
Normal Tissue
Figure 5 Cerebral cortex
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Glial cells
Staining:Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_B_9_5.jpg
Normal Tissue
Figure 6 Colon
Endocrine cells
Staining:Medium
Intensity: Moderate
Endothelial cells
Staining:Medium
Intensity: Moderate
Quantity:>75%
Fibroblasts
Staining:High
Intensity: Strong
Goblet cells
Staining:Low
Intensity: Moderate
Quantity: <25%
Mucosal lymphoid cells
Staining:High
Intensity: Strong
Quantity:>75%
Peripheral nerve/ganglion
Staining:High
Intensity: Strong
Quantity:>75%
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_8_3.jpg
Normal Tissue
Figure 7 Kidney
Bowman's capsule
Staining:Medium
Intensity: Moderate
Quantity:>75%
Collecting ducts
Staining:Medium
Intensity: Moderate
Quantity:>75%
Distal tubules
Staining:Medium
Intensity: Moderate
Quantity:>75%
Proximal tubules (microvilli)
Staining:Medium
Intensity: Moderate
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_9_5.jpg
Normal Tissue
Figure 8 Testis
Cells in seminiferous ducts
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
Leydig cells
Staining:High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_5_6.jpg
Normal Tissue
Figure 9 Prostate
Glandular cells
Staining:High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/images/70796/168931_A_2_5.jpg
RNA Expression
Figure 10 RNA cell line category: Cell line enhanced (CAPAN-2, LHCN-M2, RPTEC TERT1, U-251 MG)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas https://v21.proteinatlas.org/ENSG00000122861-PLAU
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Specifications
- Immunogen
- A synthesized peptide derived from human Urokinase
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human PLAU
- Species Reactivity
- Human
- Clone
- 10E5
- Applications
- ELISA, IHC
- Conjugate
- Unconjugated
- Related Disease
- Quebec Platelet Disorder and Alzheimer Disease
Product Property
- Purification
- Affinity purified
- Concentration
- See the COA
- Buffer
- PBS, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
- Preservative
- 0.02% sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C for short term (1-2 weeks). Aliquot and store at -20°C for long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- This antibody has been tested for use in Enzyme-linked Immunosorbent Assay, Immunohistochemistry. Specific conditions should be optimized by the end user.
IHC:1:50-1:200
Target
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Downloadable Resources
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Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Protocol & Troubleshooting
We have outlined the assay protocols, covering reagents, solutions, procedures, and troubleshooting tips for common issues in order to better assist clients in conducting experiments with our products. View the full list of Protocol & Troubleshooting.
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