Rabbit Anti-PLCB1 Recombinant Antibody (clone R39-2S-8)
CAT#: VS3-FY2183
This R39-2S-8 recombinant monoclonal antibody is specific for human PLCB1 protein, a calculated weight of 139 kDa. It cross-reacts with mouse, rat.
The following recommendations serve as a starting point for assay optimization. The actual working concentration may vary and should be determined by the user. The R39-2S-8 is recommended for detection of target protein by western blotting (dilution range 1:500-1:1000), immunohistochemistry (dilution range 1:100-1:200).
Gene Expression
Subcellular Location and Protein Expression
Figure 1 IF staining of human cell line A-431
Immunofluorescent staining of human cell line A-431 shows localization to plasma membrane.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5334/if_selected.jpg
Subcellular Location and Protein Expression
Figure 2 IHC staining of human cerebral cortex
Immunohistochemical staining shows high expression in cerebral cortex
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/5334/148012_B_8_5_val_selected.jpg
Normal Tissue
Figure 3 Cerebral cortex
Neuronal cells
Staining: High
Intensity: Strong
Quantity: 75%-25%
Location: Cytoplasmic/membranous
Neuropil
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Location: Cytoplasmic/membranous
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4275/148706_B_8_5.jpg
Normal Tissue
Figure 4 Hippocampus
Neuronal cells
Staining: High
Intensity: Strong
Quantity:>75%
Location: Cytoplasmic/membranous nuclear
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4275/148706_B_8_6.jpg
Normal Tissue
Figure 5 Kidney
Collecting ducts
Staining: Medium
Intensity: Moderate
Quantity: 75%-25%
Distal tubules
Staining: Low
Intensity: Moderate
Quantity: <25%
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/images/4275/148706_A_8_5.jpg
RNA Expression
Figure 6 RNA cell line category: Cell line enhanced (CACO-2, Hep G2)
Cell lines ordered by descending RNA expression order.
* Image credit: Image credit: Human Protein Atlas v21.proteinatlas.org/ENSG00000182621-PLCB1/subcellular
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Specifications
- Host Species
- Rabbit
- Type
- Rabbit IgG
- Specificity
- Human, Mouse and Rat PLCB1
- Species Reactivity
- Human, Mouse, Rat
- Clone
- R39-2S-8
- Applications
- Western Blot, Immunohistochemistry-Paraffin
- Conjugate
- Unconjugated
- MW
- The calculated weight of the target protein is approximately 139kDa, while the observed molecular weight stands at 100,150kDa.
- Related Disease
- Developmental And Epileptic Encephalopathy 12 and Malignant Migrating Focal Seizures Of Infancy
Product Property
- Format
- Liquid
- Concentration
- Please refer to the vial label for the specific concentration.
- Buffer
- PBS (pH 7.4), 150 mM NaCl, 50% glycerol
- Preservative
- 0.02% Sodium azide
- Storage
- Centrifuge briefly prior to opening vial. Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze/thaw cycles.
Applications
- Application Notes
- Western Blot: 1/500-1/1000
Immunohistochemistry: 1/100-1/200
Target
- Alternative Names
- Plcb; mKIAA0581; 3110043I21Rik
- Gene ID
- 23236
- UniProt ID
- D3DW12
- Cellular Localization
- Cytoplasm, Membrane, Nucleus
- Post Translation Modifications
- Palmitoylated.
Palmitoylation at Cys-17 by ZDHHC21 regulates the signaling activity of PLCB1 and the function of the endothelial barrier.
Palmitoylation by ZDHHC21 is stimulated by inflammation.
(Q9NQ66-PLCB1_HUMAN)
Modification sites at PhosphoSitePlus (Q9NQ66)
Modification sites at neXtProt (NX_Q9NQ66)
Glycosylation from GlyGen (Q9NQ66) 4 sites, 1 O-linked glycan (4 sites)
- Protein Refseq
- NP_056007.1; NP_877398.1
- Function
- Catalyzes the hydrolysis of 1-phosphatidylinositol 4,5-bisphosphate into diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) and mediates intracellular signaling downstream of G protein-coupled receptors.
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Related Signaling Pathways
cAMP Signaling Pathway
Related Diseases
Morphine Addiction
Downloadable Resources
Download resources about recombinant antibody development and antibody engineering to boost your research.
Product Notes
This is a product of Creative Biolabs' Hi-Affi™ recombinant antibody portfolio, which has several benefits including:
• Increased sensitivity
• Confirmed specificity
• High repeatability
• Excellent batch-to-batch consistency
• Sustainable supply
• Animal-free production
See more details about Hi-Affi™ recombinant antibody benefits.
Datasheet
MSDS
COA
Certificate of Analysis LookupTo download a Certificate of Analysis, please enter a lot number in the search box below. Note: Certificate of Analysis not available for kit components.
Lot Number:
Isotype Control
- CAT
- Product Name
Secondary Antibody
- CAT
- Product Name
See other products for "PLCB1"
Select a product category from the dropdown menu below to view related products.
| CAT | Product Name | Application | Type |
|---|---|---|---|
| MOB-2344CT | Recombinant Mouse anti-Human PLCB1 Monoclonal antibody (FQS4039) | IHC-P | |
| VS3-FY1831 | Rabbit Anti-Plcb1 Recombinant Antibody (clone R03-6E6) | WB | Rabbit IgG |
| VS13-YC908 | Rabbit Anti-Plcb1 Recombinant Antibody (VS13-YC908) | WB | Rabbit IgG |
| CAT | Product Name | Application | Type |
|---|---|---|---|
| VS-0525-XY5464 | Anti-PLCB1 Immunohistochemistry Kit | IHC |
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